Sample Count Frequency CD69_MFI CD69_CV CD69_MAD CD203_MFI CD203_CV CD203_MAD CD123_MFI CD123_CV CD123_MAD CD63_MFI CD63_CV CD63_MAD CD63-CD203+ CD63-CD203+_CD69_CV CD63-CD203+_CD69_MFI CD63-CD203+_CD69_MAD CD63-CD203+_CD63_CV CD63-CD203+_CD63_MFI CD63-CD203+_CD63_MAD CD63-CD203+_CD203_CV CD63-CD203+_CD203_MFI CD63-CD203+_CD203_MAD CD63-CD203+_CD123_CV CD63-CD203+_CD123_MFI CD63-CD203+_CD123_MAD CD63-CD203+_HLADR_MFI CD63+CD203+ CD63+CD203+_CD69_CV CD63+CD203+_CD69_MFI CD63+CD203+_CD69_MAD CD63+CD203+_CD63_CV CD63+CD203+_CD63_MFI CD63+CD203+_Cd63_MAD CD63+CD203+_CD203_CV CD63+CD203+_CD203_MFI CD63+CD203+_CD203_MAD CD63+CD203+_CD123_CV CD63+CD203+_CD123_MFI CD63+CD203+_CD123_MAD CD63+CD203+_HLADR_MFI CD63+CD203- CD63-CD203- CD63-CD203-_CD69_CV CD63-CD203-_CD69_MFI CD63-CD203-_CD69_MAD CD63-CD203-_CD63_CV CD63-CD203-_CD63_MFI CD63-CD203-_CD63_MAD CD63-CD203-_CD203_CV CD63-CD203-_CD203_MFI CD63-CD203-_CD203_MAD CD63-CD203-_CD123_CV CD63-CD203-_CD123_MFI CD63-CD203-_CD123_MAD CD63-CD203-_HLADR_MFI CD63-CD69+ CD63-CD69+_CD69_CV CD63-CD69+_CD69_MFI CD63-CD69+_CD69_MAD CD63-CD69+_CD63_CV CD63-CD69+_CD63_MFI CD63-CD69+_CD63_MAD CD63-CD69+_CD203_CV CD63-CD69+_CD203_MFI CD63-CD69+_CD203_MAD CD63-CD69+_CD123_CV CD63-CD69+_CD123_MFI CD63-CD69+_CD123_MAD CD63+CD69+ CD63+CD69+_CD69_CV CD63+CD69+_CD69_MFI CD63+CD69+_CD69_MAD CD63+CD69+_CD63_CV CD63+CD69+_CD63_MFI CD63+CD69+_CD63_MAD CD63+CD69+_CD203_CV CD63+CD69+_CD203_MFI CD63+CD69+_CD203_MAD CD63+CD69+_CD123_CV CD63+CD69+_CD123_MFI CD63+CD69+_CD123_MAD CD63+CD69- CD63+CD69-_CD69_CV CD63+CD69-_CD69_MFI CD63+CD69-_CD69_MAD CD63+CD69-_CD63_CV CD63+CD69-_CD63_MFI CD63+CD69-_CD63_MAD CD63+CD69-_CD203_CV CD63+CD69-_CD203_MFI CD63+CD69-_CD203_MAD CD63+CD69-_CD123_CV CD63+CD69-_CD123_MFI CD63+CD69-_CD123_MAD CD63-CD69- CD63-CD69-_CD69_CV CD63-CD69-_CD69_MFI CD63-CD69-_CD69_MAD CD63-CD69-_CD63_CV CD63-CD69-_CD63_MFI CD63-CD69-_CD63_MAD CD63-CD69-_CD203_CV CD63-CD69-_CD203_MFI CD63-CD69-_CD203_MAD CD63-CD69-_CD123_CV CD63-CD69-_CD123_MFI CD63-CD69-_CD123_MAD 1: 1 RPMI pre 2103 1.63 124 212 237 1452 91.9 132 43189 31.4 148 402 127 66.7 55.7 142 130 230 58.4 422 69 70.2 2293 137 31.7 43831 148 237 0.14 28.4 12517 146 21.3 14096 145 21.1 14860 147 27.8 34199 148 14786 0 44.1 162 114 250 34 374 63.9 30.3 821 123 30.9 42424 148 226 27 48.1 796 144 52.4 427 69.1 82.1 1521 133 27.8 47643 148 0.14 28.4 12517 146 21.3 14096 145 21.1 14860 147 27.8 34199 148 0 • • • • • • • • • • • • 72.9 233 42.8 507 50.7 391 65.9 91.4 1422 132 32.2 41669 148 2: 2 fMLP pre 2335 1.64 124 260 238 2230 110 137 42747 32.5 148 622 273 81.8 66 150 116 245 132 535 82.1 80.9 2841 139 32.2 43383 148 220 6.68 137 320 165 43 17454 146 44.7 10808 146 33.1 43991 148 308 0 27.4 427 111 249 46.7 419 76.4 27.1 870 126 32.7 40965 148 253 23.9 154 804 143 87.6 509 82.7 91 1961 136 31.7 46505 148 3.6 80.6 1209 138 42.6 18947 146 48 10953 146 30.6 47549 148 4.03 151 78.8 270 50.5 12938 145 42.7 9874 146 33.3 40161 148 68.5 253 37.7 787 97.9 478 79.3 97.3 1972 136 32.1 41429 148 3: 3 IgE1 pre 2043 1.43 118 209 245 2546 100 139 42633 31 148 482 402 76.7 75.5 154 116 246 76.9 478 78.1 78.7 3492 141 30.5 42542 148 232 1.57 165 479 150 47.8 24510 146 51.4 13402 147 43.5 44694 148 470 0 23 150 112 252 35.2 388 71.6 31.4 830 126 31.7 42797 148 245 26.9 46 785 156 59.9 483 78.5 89.8 2385 139 29.7 44972 148 1.08 123 1887 141 50.5 22590 146 57.9 13371 147 41.3 41324 148 0.59 -3029 -22.1 -379 57.1 22753 147 29.9 13856 147 43.1 51296 148 71.5 269 34.5 950 66.3 443 75.2 95.4 2512 139 30.9 41742 148 4: 4 IgE2 pre 1492 1.4 116 183 243 1715 97.8 135 41312 30.4 148 515 192 81.1 64.3 156 117 239 37.1 531 82.9 72.3 2551 138 30.2 42149 148 221 0.47 82.8 2028 144 45.6 14846 145 51.3 13388 147 40.7 29168 147 4378 0 35.2 173 109 257 31.2 468 77.4 30.5 822 124 30.4 40011 148 235 26.5 53.7 806 150 34.6 551 84 95.3 1690 135 29.6 43313 148 0.4 67.8 3023 144 46.4 15379 145 51 14037 147 41.4 30236 147 0.067 • 211 225 • 12014 144 • 10080 146 • 23511 147 73.1 267 34.9 845 36.3 493 79.7 88.1 1702 135 30.5 40701 148 5: 5 IgE3 pre 1408 1.31 92.2 205 284 1480 98.3 135 39608 28.7 148 517 123 85.6 57.8 190 95.8 278 45.7 545 87.8 73.3 2328 139 28.1 40829 148 241 0.14 7.93 6598 144 8 18255 146 12.8 21690 147 26.8 30865 148 23275 0 42 179 85.7 290 31.8 476 83.1 31.6 799 126 29.1 38021 148 213 20.6 59.6 788 163 52.2 561 88.9 95.2 1626 136 27.4 42867 148 0.14 7.93 6598 144 8 18255 146 12.8 21690 147 26.8 30865 148 0 • • • • • • • • • • • • 79.3 276 33 2289 37.7 503 84.8 90 1438 135 28.7 38820 148 6: 6 RPMI post 1391 1.31 104 637 267 1181 122 130 39382 27.9 148 533 276 82.5 43.8 759 108 254 35.5 562 84.8 55.7 1960 136 27.3 40705 148 231 0.36 132 1946 147 65.4 21570 146 89.5 14201 146 15.7 63652 148 5094 0 55.9 161 98.6 273 32.7 500 80 31 789 123 27.8 38256 148 195 23.7 370 785 145 35.5 566 85.6 79.1 1222 130 25.8 41319 148 0.29 111 3565 144 52.9 27193 147 80.1 17316 147 14.8 66143 148 0.072 • 199 230 • 8566 143 • 6444 145 • 54592 148 76 269 34.5 859 34 514 81.1 71.8 1155 130 28.2 38709 148 7: 7 fMLP post 1356 1.42 106 398 264 2234 104 138 41880 30.3 148 805 288 93.8 65.7 170 93.2 291 107 675 95 72.6 2849 140 30.3 41812 148 184 7.6 376 240 203 50.7 23862 147 50.8 9349 146 35.2 43237 148 354 0.15 26.5 153 110 243 32.4 496 86.6 29.4 840 126 28.7 41733 148 218 21.9 42.7 798 151 78.6 623 94.2 85.6 1977 137 27.5 44932 148 3.32 243 1386 140 52.4 25022 147 57.8 9209 146 41.4 45860 148 5.09 222 46.4 402 56.6 19249 146 49.8 8520 146 26 40621 148 69.7 303 28.2 -5144 88.9 602 91.2 88.4 1973 137 30.4 40878 148 8: 8 IgE1 post 1315 1.31 98.5 182 271 2325 96.9 138 41086 27.8 148 640 331 87.4 73.2 173 92.4 273 70.7 638 88.9 76.3 3227 140 27.4 40944 148 220 1.83 98.7 723 139 45.7 23275 146 42.4 11397 146 36.1 44637 148 443 0 24.9 159 98.8 275 33.5 507 81.3 30.4 814 123 28.3 41255 148 211 22.4 44.9 759 152 69.5 647 89.5 95.1 2230 138 25.5 44092 148 1.37 72 1671 139 40.5 26329 147 42.4 12049 146 40.1 44672 148 0.68 340 25.4 -817 65.1 11619 144 43.4 10777 146 24.9 39620 148 75.6 295 29.7 2298 49.1 585 86.3 90.7 2256 138 27.9 40190 148 9: 9 IgE2 post 1400 1.48 103 379 265 1752 98.6 134 42199 30.4 148 602 305 84.5 64.3 156 110 248 55.7 626 86.3 73.8 2672 138 30.4 42083 148 208 0.5 150 182 262 32.7 31528 147 30 14603 147 30.1 47824 148 215 0 35.2 574 91.2 292 28.8 532 80.1 31.3 803 122 30.4 42336 148 209 23.3 214 774 146 33.5 640 88.2 87.1 1682 134 29.9 44462 148 0.21 59.4 1529 140 17.9 41461 147 40.9 13609 146 15.5 50781 148 0.36 758 13.5 526 51.7 19465 147 40.6 12805 147 47.7 39962 148 76.1 261 36.1 1067 48.3 575 82.7 94.6 1748 134 30.3 41519 148 10: 10 IgE3 post 1382 1.51 102 234 275 1223 108 129 40490 28.9 148 470 187 72.5 43.6 209 109 271 69.4 490 75.1 64.8 2172 136 28.2 41768 148 194 0.29 19.9 7124 146 17.4 18545 146 26.1 20625 147 22.8 29795 148 20860 0 56.1 178 94.1 283 30.6 447 70.2 32.3 780 122 29.2 39585 148 198 22.1 54 746 143 36.1 480 75.2 84.7 1297 130 28.6 42367 148 0.36 22.8 7635 146 35.5 15397 146 37.8 17637 147 20.1 29600 147 0 • • • • • • • • • • • • 77.6 251 38.3 565 41.1 460 71.4 86.9 1188 129 28.8 40030 148 11: 11 RPMI 4-hour 1417 0.88 51.8 424 622 536 202 107 16528 26.2 147 282 305 52.5 4.38 326 78.8 614 182 443 56.6 69.6 2056 136 25.5 17035 147 175 0.56 61.2 5087 147 52.8 12640 145 55.6 14701 147 51.5 23801 147 18330 0 95.1 330 48.1 680 35.8 271 52.5 42.7 496 105 25.4 16469 147 102 10.2 149 692 143 98 309 53.9 101 579 110 31 17452 147 0.78 62.9 4492 147 57.8 10717 144 60.1 12512 146 56.2 20344 147 0.14 578 22.1 878 6.51 5928 142 22.8 2700 140 10.3 15837 147 88.9 357 21.9 -1505 49.8 270 52.1 62.7 516 106 24.5 16396 147 12: 12 fMLP 4-hour 1737 1.08 44.4 942 781 727 146 116 13367 29.3 147 355 241 55.5 18.3 236 44.9 668 125 770 76.8 39.5 2069 137 28.1 13031 147 81 3.97 293 107 328 26.9 10609 145 109 3733 142 48.4 14783 147 151 0.058 77.7 1174 41.4 949 134 252 55.4 45.4 529 109 27.4 13380 147 99.7 6.79 463 647 141 169 330 57.1 80.1 709 116 26.4 14347 147 0.98 121 1634 140 46.5 10642 145 103 5472 143 52.4 19379 147 5.47 372 21.4 -7364 27.1 8497 144 51.6 2850 141 33.7 13437 147 86.8 337 24.3 -9205 154 286 55.1 83.2 656 114 27.6 13233 147 13: 13 IgE1 4-hour 1703 0.79 51.2 539 525 1291 132 132 13788 27.8 147 379 248 52.3 45.1 527 40 874 190 381 55.5 67.4 2183 137 23.2 12991 147 92.5 7.57 246 138 251 31.2 11417 145 104 4292 142 41.7 16268 147 239 0.12 47.2 680 50.2 491 51.2 225 54.5 40.1 659 118 24.7 14200 147 94.4 6.75 272 651 143 128 274 52.1 201 1101 128 24.7 14653 147 1.88 93 2014 144 38.3 13690 145 74.9 9074 146 47.3 20567 147 7.63 219 46.9 614 27.4 9489 144 53.6 3317 142 28.7 14705 147 83.7 327 25.8 2511 126 275 52 68.5 1152 130 24.3 13518 147 14: 14 IgE2 4-hour 973 1 102 188 258 1619 103 134 40667 28.8 148 636 281 83.5 57.5 194 113 235 50.8 660 86.8 73.3 2778 139 28.1 40994 148 236 1.03 159 149 282 30 23683 146 75.6 6785 144 45.2 43191 148 142 0 41.5 173 86.2 327 28.9 556 79.8 33.6 756 122 29.1 40158 148 215 23.6 73.3 748 149 35.7 651 87.4 102 1581 133 28.9 44284 148 0.41 67.8 1218 143 41.7 24447 146 82.8 6328 145 57.3 34903 148 0.62 861 -8.89 -410 21.1 23187 146 78.6 7108 144 38.7 49786 148 75.3 273 33.6 1319 49.2 603 82.5 98.2 1600 134 27.7 39562 148 15: 15 IgE3 4-hour 1703 1.07 96.4 271 278 1190 81.9 130 40638 29.2 148 500 93.5 75.2 44.9 330 96.7 292 40.6 547 79 54 2031 136 29.1 41529 148 196 0.12 30.3 5326 146 29.7 13587 145 35 16017 147 17.6 27451 148 17579 0 55 169 95.1 275 31.8 462 72 33.4 772 123 29.1 39958 148 204 23 131 780 149 41.4 532 78.6 77.7 1176 130 27.7 42473 148 0.12 30.3 5326 146 29.7 13587 145 35 16017 147 17.6 27451 148 0 • • • • • • • • • • • • 76.9 293 30 6942 36.3 489 74.7 73.5 1189 130 29.6 40130 148 Mean 1584 1.28 95.6 351 337 1567 113 131 35968 29.4 148 516 245 75.4 52 258 97.4 351 85.1 554 79 68.2 2500 138 28.7 36375 148 198 2.19 132 2864 187 36.5 18658 146 53.3 12657 146 34.4 35837 148 7108 0.022 45.8 323 89.7 359 41.2 425 72.3 33.4 759 121 29 35437 148 195 20.6 145 757 148 67.5 506 77.7 96.5 1516 131 28.1 38379 148 1.01 79.4 3714 143 38.8 19850 146 53.7 12942 146 35.4 36258 148 1.65 52.4 57.6 -530 40.3 13973 145 45.9 8030 145 31.8 34866 148 76.8 284 32.4 339 64.4 464 74.3 85.4 1499 131 28.9 35235 148 StdDev 356 0.26 25.9 215 166 583 29.5 8.69 11151 1.64 0.41 133 85.4 13.1 19.6 172 25.7 198 50.9 105 11.3 10.9 466 1.67 2.36 11466 0.41 49.4 2.82 106 3694 62.7 15.3 5965 0.7 29.3 5160 1.77 10.9 13089 0.46 8978 0.049 20.1 290 24.2 201 26.4 103 11 5.19 111 6.19 2.15 10848 0.41 52.1 6.84 131 52.7 5.96 39.5 123 13.6 29.9 514 8.06 2.03 11973 0.41 1.12 57.3 3188 2.91 14.2 8194 0.94 24.2 4359 1.08 14.6 13087 0.49 2.55 1182 77.8 2321 20.1 6120 1.62 15.9 3911 2.46 11.1 15324 0.5 5.87 34.5 5.68 3617 36.3 114 12.6 11.1 553 9.04 2.35 10853 0.41