LOCUS New_DNA 3949 bp ds-DNA circular 01-FEB-2009 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT Insert from C:/Users/JCAnderson/Documents/Sequences/pBca1256.ape 160 to 1270 Backbone from C:/Users/JCAnderson/Documents/Sequences/pBca1254CA.ape 1003 to 23 COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_recomb 1..17 /label=AttL1 /ApEinfo_fwdcolor=plum /ApEinfo_revcolor=plum CDS complement(1996..2856) /label=AmpR /ApEinfo_fwdcolor=#f8c887 /ApEinfo_revcolor=#f8c887 misc_feature 2995..3000 /label=PstI /ApEinfo_fwdcolor=#0080ff /ApEinfo_revcolor=#0080ff misc_feature 997..1002 /label=BamHI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_recomb 3001..3034 /label=LoxP /ApEinfo_fwdcolor=steel blue /ApEinfo_revcolor=orange misc_feature 3035..3040 /label=XhoI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 87..92 /label=EcoRI /ApEinfo_fwdcolor=#0080ff /ApEinfo_revcolor=#0080ff misc_recomb 3942..3949 /label=AttR1 /ApEinfo_fwdcolor=plum /ApEinfo_revcolor=plum misc_feature 868..996 /label=dblTerm /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_recomb complement(1112..1126) /label=AttR2 /ApEinfo_fwdcolor=plum /ApEinfo_revcolor=plum misc_feature 102..155 /label=P_tet of R0040 /ApEinfo_fwdcolor=#dfd0df /ApEinfo_revcolor=#dfd0df misc_feature 96..101 /label=BglII /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#00ff80 misc_feature complement(3162..3821) /label=CmR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 181..858 /label=mRFP /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=#ff0080 misc_feature 1223..1905 /label=colE1 ori /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=#c0c0c0 misc_feature complement(1075..1094) /label=G00101 /ApEinfo_fwdcolor=#99e4fb /ApEinfo_revcolor=#99e4fb misc_feature 26..47 /label=CA998 /ApEinfo_fwdcolor=#9bd1f9 /ApEinfo_revcolor=#9bd1f9 misc_feature 1102..1111 /label=attL2 /ApEinfo_fwdcolor=#e9b4fa /ApEinfo_revcolor=#e9b4fa ORIGIN 1 GTACAAAAAA GCAGGCTCCG AATTGgtatc acgaggcaga atttcagata aaaaaaatcc 61 ttagctttcg ctaaggatga tttctgGAAT TCATGAGATC TTCCCTATCA GTGATAGAGA 121 TTGACATCCC TATCAGTGAT AGAGATACTG AGCACGGATC TGAAAGAGGA GAAAGGATCT 181 ATGGCAAGTA GCGAAGACGT TATCAAAGAG TTCATGCGTT TCAAAGTTCG TATGGAAGGT 241 TCCGTTAACG GTCACGAGTT CGAAATCGAA GGTGAAGGTG AAGGTCGTCC GTACGAAGGT 301 ACCCAGACCG CTAAACTGAA AGTTACCAAA GGTGGTCCGC TGCCGTTCGC TTGGGACATC 361 CTGTCCCCGC AGTTCCAGTA CGGTTCCAAA GCTTACGTTA AACACCCGGC TGACATCCCG 421 GACTACCTGA AACTGTCCTT CCCGGAAGGT TTCAAATGGG AACGTGTTAT GAACTTCGAA 481 GACGGTGGTG TTGTTACCGT TACCCAGGAC TCCTCCCTGC AAGACGGTGA GTTCATCTAC 541 AAAGTTAAAC TGCGTGGTAC CAACTTCCCG TCCGACGGTC CGGTTATGCA GAAAAAAACC 601 ATGGGTTGGG AAGCTTCCAC CGAACGTATG TACCCGGAAG ACGGTGCTCT GAAAGGTGAA 661 ATCAAAATGC GTCTGAAACT GAAAGACGGT GGTCACTACG ACGCTGAAGT TAAAACCACC 721 TACATGGCTA AAAAACCGGT TCAGCTGCCG GGTGCTTACA AAACCGACAT CAAACTGGAC 781 ATCACCTCCC ACAACGAAGA CTACACCATC GTTGAACAGT ACGAACGTGC TGAAGGTCGT 841 CACTCCACCG GTGCTTAATA AGGATCTCCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA 901 AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCTAC TAGAGTCACA 961 CTGGCTCACC TTCGGGTGGG CCTTTCTGCG TTTATAGGAT CCtaaCTCGA cgtgcaggct 1021 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 1081 tcaaaggcgg taatCAATTC GACCCAGCTT TCTTGTACAA AGTGGTTGAT CCTTACAGAT 1141 CCcggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 1201 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc 1261 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 1321 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 1381 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 1441 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 1501 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 1561 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 1621 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 1681 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 1741 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 1801 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 1861 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgaACTAG Caaggatctt 1921 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 1981 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 2041 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 2101 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 2161 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 2221 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 2281 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 2341 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 2401 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 2461 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 2521 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 2581 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 2641 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 2701 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 2761 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 2821 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 2881 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 2941 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcctCTGCAG 3001 ataacttcgt atagcataca ttatacgaag ttatctcgag gcttggattc tcaccaataa 3061 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta 3121 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac 3181 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg 3241 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg 3301 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg 3361 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg 3421 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc 3481 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa 3541 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata 3601 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac 3661 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg 3721 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg 3781 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct 3841 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag 3901 ttggaaCCTC TTACGTGCCC GATCAAGaat tgCCATTATC AACAAGTTT //