LOCUS pKn001 3712 bp DNA circular 2-JUL-2008 DEFINITION ATCC 37253: very usable general purpose vector. KEYWORDS ATCC. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|486739835| COMMENT VNTDBDATE|486828895| COMMENT LSOWNER| COMMENT VNTNAME|pKn001| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTAUTHOREML|mgethers@mit.edu| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria FEATURES Location/Qualifiers promoter complement(3547..3547) /vntifkey="30" /label=P(BLA) /note=" bla gene promoter " CDS complement(2655..3512) /vntifkey="4" /label=AP(R) /note=" bla gene- Ap(r) determinant " promoter complement(1533..1533) /vntifkey="30" /label=P(LAC) /note=" lac promoter " rep_origin 1893..1893 /vntifkey="33" /label=ORI /note=" RNaseH cleavage point " CDS complement(149..1495) /vntifkey="4" /label=ALPHA /note=" lacZ gene fragment coding for LacZ alpha peptide " misc_feature 1503..1526 /vntifkey="21" /label=M13\Reverse\Primer\Binding\Site misc_feature 352..375 /vntifkey="21" /label=M13\Forward\Primer\Binding\Site primer_bind 437..456 /vntifkey="28" /label=BT2724 misc_feature 437..1462 /vntifkey="21" /label=HmgA\Downstream\Fragment\(cut\with\NcoI) BASE COUNT 813 a 988 c 1092 g 819 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg 421 actctagagg atccccctgg tcgttgtcgt cgtagagata cggcagcggc cgcgggtcgc 481 cttcggggcg cgctggctgg gcacggcgga acggctccag ggcttcggcg gtgaccaccc 541 agggcgagac gctggtaatg aagctcttcg acaggaacgg gccgagcggt tggtattccc 601 aggcctggat gtcgcgcgcc gaccagtcgt tgagcaggca gaagccggcg atgtgctcgg 661 cggcgcggga aaccgggatg gcttcgccga gggcgttgcc ctggccgatc cagatgccca 721 gctccagttc gtagtccagg cgcgcgcagg ggccgaacac cggctcgctg gcgccggccg 781 gcagggtctg gccgttgggc cggcgcactt cgctgccgga gacgcgcacg gtcgaggcgc 841 ggccgtggta gccgatgggc acgtgcttgt agttgggcag cagtgggttg tccgggcgga 901 acagcttgcc gacgttctcg gcatggcgga tgccgacgta gaagtcggtg tagtcgccga 961 tcctggccgg taggtgcatt tggcactcgg cggccggatg gagcagttcc gggcgctccg 1021 cggcgccttc gccgagcagc tccagcaggc gctggcgcag cgccacgcgg gccggcttgc 1081 cgagggcgaa gaaggcgttc agcgcgccgc cgcgggtggc ttcggcggct tcgccggcgg 1141 caccctcgaa cagcccggcg gccagcgccg cgtccaggtc gaggatcgag tcgccgatgg 1201 ccacgccgca acgcgggctc aggccggcac ggctgaagat gcccagcggc aggttttgca 1261 ggggaaagtc ggcatgccca ttggcggagg cgacccagct acgagtgggg gttggctggt 1321 tcatggggtt atctccgttg cgggttgaag gtggatacga gcgacgccca gcaggcatcg 1381 tagtcattct gcagggccgg cgtctcgagg gcggcacggc tcggccggag gacctggctg 1441 gtctcgaaca tgaaggccat gggggtaccg agctcgaatt cgtaatcatg gtcatagctg 1501 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 1561 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 1621 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 1681 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 1741 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 1801 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 1861 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 1921 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 1981 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 2041 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 2101 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 2161 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 2221 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 2281 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 2341 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 2401 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 2461 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 2521 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 2581 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 2641 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 2701 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 2761 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 2821 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 2881 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 2941 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 3001 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 3061 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 3121 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 3181 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 3241 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 3301 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 3361 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 3421 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 3481 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 3541 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 3601 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt 3661 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc //