LOCUS                  New_DNA        2745 bp ds-DNA   linear       31-MAR-2010
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             1441..2100
                     /label=AmpR
                     /ApEinfo_fwdcolor=#f8c887
                     /ApEinfo_revcolor=#f8c887
     misc_feature    complement(2205..2584)
                     /label=R6K
                     /ApEinfo_fwdcolor=#8080ff
                     /ApEinfo_revcolor=#8080ff
     misc_feature    265..924
                     /label=CmR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
     misc_feature    78..97
                     /label=G00101
                     /ApEinfo_fwdcolor=#8080ff
                     /ApEinfo_revcolor=#8080ff
     misc_feature    2675..2696
                     /label=ca998
                     /ApEinfo_fwdcolor=#008040
                     /ApEinfo_revcolor=#008040
     misc_feature    2467..2472
                     /label=HindIII
                     /ApEinfo_fwdcolor=#408080
                     /ApEinfo_revcolor=#9ecfcf
     misc_feature    779..784
                     /label=NcoI
                     /ApEinfo_fwdcolor=#800000
                     /ApEinfo_revcolor=green
     misc_feature    2591..2596
                     /label=NcoI(1)
                     /ApEinfo_label=NcoI
                     /ApEinfo_fwdcolor=#800000
                     /ApEinfo_revcolor=green
     misc_feature    1070..1075
                     /label=PstI
                     /ApEinfo_fwdcolor=#0080ff
                     /ApEinfo_revcolor=#0080ff
     misc_feature    2736..2741
                     /label=EcoRI
                     /ApEinfo_fwdcolor=#0080ff
                     /ApEinfo_revcolor=#0080ff
     misc_feature    1064..1069
                     /label=XhoI
                     /ApEinfo_fwdcolor=#008000
                     /ApEinfo_revcolor=#008000
     misc_feature    complement(13..18)
                     /label=BseRI (F green, R blue)
                     /ApEinfo_fwdcolor=#6bff9c
                     /ApEinfo_revcolor=#7bd5ff
     misc_feature    complement(1091..1096)
                     /label=BseRI (F green, R blue)(1)
                     /ApEinfo_label=BseRI (F green, R blue)
                     /ApEinfo_fwdcolor=#6bff9c
                     /ApEinfo_revcolor=#7bd5ff
ORIGIN
        1 GATCCtaaCT CGCTCCTCag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
       61 tgcggcgagc ggtatcagct cactcaaagg cggtaatCAA TTCGACCCAG CTTTCTTGTA
      121 CAAAGTGGTT GATCcttacA GATCCcggtt atcGGATCTt tgatcgggca cgtaagaggt
      181 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
      241 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
      301 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
      361 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
      421 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
      481 tttcgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
      541 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
      601 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
      661 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
      721 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
      781 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
      841 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
      901 gatgagtggc agggcggggc gtaatttgat atcgagctcg cttggactcc tgttgataga
      961 tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc
     1021 gttttttatt ggtgagaatc caagcctcGG ATCTCTGAAG actctcgagC TGCAGtctag
     1081 GGactcacag CTCCTCGGAT CTcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     1141 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1201 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1261 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1321 acGCTGGTGA AAGTAAAAGA TGCAGAGGAT CAGTTGGGTG CACGAGTGgg ttacatcgaa
     1381 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1441 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1501 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1561 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1621 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1681 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     1741 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     1801 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     1861 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     1921 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     1981 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2041 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2101 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2161 tttaaaagga tctaGGTGAA GATCCTTTTT GATAATCGGA TCTaGCAGTT CAACCTGTTG
     2221 ATAGtacgta ctaagctctc atgtttcacg tactaagctc tcatgtttaa cgtactaagc
     2281 tctcatgttt aacgaactaa accctcatgg ctaacgtact aagctctcat ggctaacgta
     2341 ctaagctctc atgtttcacg tactaagctc tcatgtttga acaataaaat taatataaat
     2401 cagcaactta aatagcctct aaggttttaa gttttataag aaaaaaaaga atatataagg
     2461 cttttaaagc ttttaaggtt taacggttgt ggacaacaag ccagggatgt aacgcactga
     2521 gaagccctta gagcctctca aagcaatttt gagtgacaca ggaacactta acggctgaca
     2581 tgggaattag ccatgggccc gtgcgaatca cGGATCTccc gatcaaGaat tgCCATTATC
     2641 AACAAGTTTG TACAAAAAAG CAGGCTCCGA ATTGgtatca cgaggcagaa tttcagataa
     2701 aaaaaatCCT TAGCTTTCGC TAAGGATCTG AAGTGgaatt catgA 
//