LOCUS New_DNA 3884 bp ds-DNA circular 01-FEB-2009 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT Insert from C:/Users/JCAnderson/Documents/Sequences/pBca1256.ape 160 to 1270 Backbone from C:/Users/JCAnderson/Documents/Sequences/pBca1254KC.ape 1003 to 23 COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_recomb 1..17 /label=AttL1 /ApEinfo_fwdcolor=plum /ApEinfo_revcolor=plum misc_recomb complement(1112..1126) /label=AttR2 /ApEinfo_fwdcolor=plum /ApEinfo_revcolor=plum misc_feature 2017..2676 /label=CmR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan misc_feature 997..1002 /label=BamHI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_recomb 3877..3884 /label=AttR1 /ApEinfo_fwdcolor=plum /ApEinfo_revcolor=plum misc_feature 87..92 /label=EcoRI /ApEinfo_fwdcolor=#0080ff /ApEinfo_revcolor=#0080ff misc_feature 868..996 /label=dblTerm /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 102..155 /label=P_tet of R0040 /ApEinfo_fwdcolor=#dfd0df /ApEinfo_revcolor=#dfd0df misc_feature 2838..2843 /label=XhoI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 1223..1905 /label=colE1 ori /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=#c0c0c0 misc_feature 96..101 /label=BglII /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#00ff80 misc_feature 1137..1141 /label=BglII(1) /ApEinfo_label=BglII /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#00ff80 misc_feature 3862..3862 /label=EcoRI(1) /ApEinfo_label=EcoRI /ApEinfo_fwdcolor=#0080ff /ApEinfo_revcolor=#0080ff misc_feature 181..858 /label=mRFP /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=#ff0080 misc_feature 2992..3807 /label=KnR Kanamycin Resistance /ApEinfo_fwdcolor=#548d5c /ApEinfo_revcolor=#548d5c misc_feature complement(1075..1094) /label=G00101 /ApEinfo_fwdcolor=#99e4fb /ApEinfo_revcolor=#99e4fb misc_feature 2798..2803 /label=PstI /ApEinfo_fwdcolor=#0080ff /ApEinfo_revcolor=#0080ff misc_feature 26..47 /label=CA998 /ApEinfo_fwdcolor=#9bd1f9 /ApEinfo_revcolor=#9bd1f9 misc_recomb 2804..2837 /label=LoxP /ApEinfo_fwdcolor=steel blue /ApEinfo_revcolor=orange misc_feature 1102..1111 /label=attL2 /ApEinfo_fwdcolor=#e9b4fa /ApEinfo_revcolor=#e9b4fa misc_feature 1142..1142 /label=BamHI(1) /ApEinfo_label=BamHI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 ORIGIN 1 GTACAAAAAA GCAGGCTCCG AATTGgtatc acgaggcaga atttcagata aaaaaaatcc 61 ttagctttcg ctaaggatga tttctgGAAT TCATGAGATC TTCCCTATCA GTGATAGAGA 121 TTGACATCCC TATCAGTGAT AGAGATACTG AGCACGGATC TGAAAGAGGA GAAAGGATCT 181 ATGGCAAGTA GCGAAGACGT TATCAAAGAG TTCATGCGTT TCAAAGTTCG TATGGAAGGT 241 TCCGTTAACG GTCACGAGTT CGAAATCGAA GGTGAAGGTG AAGGTCGTCC GTACGAAGGT 301 ACCCAGACCG CTAAACTGAA AGTTACCAAA GGTGGTCCGC TGCCGTTCGC TTGGGACATC 361 CTGTCCCCGC AGTTCCAGTA CGGTTCCAAA GCTTACGTTA AACACCCGGC TGACATCCCG 421 GACTACCTGA AACTGTCCTT CCCGGAAGGT TTCAAATGGG AACGTGTTAT GAACTTCGAA 481 GACGGTGGTG TTGTTACCGT TACCCAGGAC TCCTCCCTGC AAGACGGTGA GTTCATCTAC 541 AAAGTTAAAC TGCGTGGTAC CAACTTCCCG TCCGACGGTC CGGTTATGCA GAAAAAAACC 601 ATGGGTTGGG AAGCTTCCAC CGAACGTATG TACCCGGAAG ACGGTGCTCT GAAAGGTGAA 661 ATCAAAATGC GTCTGAAACT GAAAGACGGT GGTCACTACG ACGCTGAAGT TAAAACCACC 721 TACATGGCTA AAAAACCGGT TCAGCTGCCG GGTGCTTACA AAACCGACAT CAAACTGGAC 781 ATCACCTCCC ACAACGAAGA CTACACCATC GTTGAACAGT ACGAACGTGC TGAAGGTCGT 841 CACTCCACCG GTGCTTAATA AGGATCTCCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA 901 AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCTAC TAGAGTCACA 961 CTGGCTCACC TTCGGGTGGG CCTTTCTGCG TTTATAGGAT CCtaaCTCGA cgtgcaggct 1021 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 1081 tcaaaggcgg taatCAATTC GACCCAGCTT TCTTGTACAA AGTGGTTGAT CcttacAGAT 1141 CCcggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 1201 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc 1261 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 1321 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 1381 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 1441 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 1501 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 1561 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 1621 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 1681 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 1741 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 1801 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 1861 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgaACTAG Cttgatcggg 1921 cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt 1981 gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat 2041 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt 2101 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta 2161 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat 2221 gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt 2281 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa 2341 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt 2401 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat 2461 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc 2521 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg 2581 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta 2641 caacagtact gcgatgagtg gcagggcggg gcgtaatttg atatcgagct cgcttggact 2701 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg 2761 ttgccgccgg gcgtttttta ttggtgagaa tccaagcCTG CAGataactt cgtatagcat 2821 acattatacg aagttatctc gagctgatcc ttcaactcag caaaagttcg atttattcaa 2881 caaagccacg ttgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca 2941 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat 3001 attcaacggg aaacgtcttg ctccaggccg cgattaaatt ccaacatgga tgctgattta 3061 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg 3121 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat 3181 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc 3241 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggg 3301 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg 3361 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc 3421 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg 3481 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat 3541 aagcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac 3601 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca 3661 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta 3721 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt 3781 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag 3841 agcattacgc tgacttgacg gGaattgCCA TTATCAACAA GTTT //