LOCUS       pKGC438                 2844 bp ds-DNA     linear       06-FEB-2012
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     1..23
                     /label=T7 R0085
                     /ApEinfo_fwdcolor=#bd3e81
                     /ApEinfo_revcolor=#bd3e81
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(1006..1688)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    912..917
                     /label=EcoRI
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2676..2681
                     /label=SpeI
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#00ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1820..1825
                     /label=XhoI
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    39..44
                     /label=XbaI
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#00ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    81..911
                     /label=CmR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC
       61 TTTAAGAAGG AGATATACAT atgagtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttCCATG GAGCGAGAAA AAAATCACTG GATATACCac cgttgatata tcccaatggc
      301 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
      361 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
      421 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggagttc cgtatggcaa
      481 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
      541 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
      601 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
      661 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
      721 atttaaacgt ggccaatatg gacaaCTTCT TCGCCCCCGT TTTCACGATG GGCAAATATT
      781 ATACGCAAGg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg
      841 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gaGTGGCAGG
      901 GCGGGGCGTA AGAATTCGGT GAACGATATC TGCTTTTCTT CGCGAATtaa ttccgcttcg
      961 caACATGTga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1021 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1081 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1141 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1201 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1261 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1321 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1381 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1441 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     1501 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1561 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1621 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1681 ggTCATGAgt tgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     1741 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
     1801 ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat
     1861 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt
     1921 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     1981 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     2041 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggga
     2101 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     2161 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     2221 atcgcgtatt tcgtTTAgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     2281 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     2341 agcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     2401 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     2461 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     2521 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     2581 atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga
     2641 gcattacgct gacttgacgg gacggcggct ttgttACTAG Tcgttcatcc atagttgcct
     2701 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2761 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2821 ccggaagggc CGATCCCGCG AAAT   
//