ID pCJW93 standard; circular DNA; ; 8663 BP. DE pCJW93 Cloning plasmid with an inducible promoter and HIS tag for use with Streptomyces CC This file is created in Vector NTI by Jim Hunter, e-mail: J.Hunter@uea.ac.uk CC http://www.invitrogen.com/ CC LSOWNER| CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter complement(5213..5327) FT /vntifkey="30" FT /label=tipA FT /note="tipA - Inducible promoter with Thiostrepton" FT terminator complement(5342..5531) FT /vntifkey="43" FT /label=t0 FT /note="t0 - Terminator" FT terminator complement(4734..5108) FT /vntifkey="43" FT /label=tfd FT /note="tfd - Terminator" FT rep_origin complement(1382..4351) FT /vntifkey="33" FT /label=oriV FT /note="oriV strep - Streptomyces origin of replication" FT CDS complement(275..1084) FT /vntifkey="4" FT /label=tsr FT /note="tsr - Thiostrepton Resistance Gene" FT CDS 6787..7563 FT /vntifkey="4" FT /label=acc(3)IV FT /note="acc(3)IV - Apramycin Resistance Gene" FT misc_feature complement(5148..5207) FT /vntifkey="21" FT /label=HIS\Tag FT /note="His Tag from pIJ4123" FT rep_origin 8398..8509 FT /vntifkey="33" FT /label=oriT FT /note="oriT" SQ Sequence 8663 BP; 1618 A; 2865 C; 2654 G; 1526 t; caagctaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc 60 caacttaatc gccttgcagc acatccccct ttcgccaggc tcgcccggcc gccggtcccc 120 ctgccgtccg gcccgtccca gaggcagcgt cggcggctcc tgcctccccc gcccggcgcc 180 gaccgggacc cgcaaacccc ttgatccgct gtcgggggtg atcatcactg acgaatcgag 240 gtcgaggaac cgagcgtccg aggaacagag gcgcttatcg gttggccgcg agattcctgt 300 cgatcctctc gtgcagcgcg attccgaggg aaacggaaac gttgagagac tcggtctggc 360 tcatcatggg gatggaaacc gaggcggaag acgcctcctc gaacaggtcg gaaggcccac 420 ccttttcgct gccgaacagc aaggccagcc gatccggatt gtccccgagt tccttcacgg 480 aaatgtcgcc atccgccttg agcgtcatca gctgcatacc gctgtcccga atgaaggcga 540 tggcctcctc gcgaccggag agaacgacgg gaagggagaa gacgtaacct cggctggccc 600 tttggagacg ccggtccgcg atgctggtga tgtcactgtc gaccaggatg atccccgacg 660 ctccgagcgc gagcgacgtg cgtactatcg cgccgatgtt cccgacgatc ttcaccccgt 720 cgagaacgac gacgtcccca cgccggctcg cgatatcgcc gaacctggcc gggcgaggga 780 cgcgggcgat gccgaatgtc ttggccttcc gctccccctt gaacaactgg ttgacgatcg 840 aggagtcgat gaggcggacc ggtatgttct gccgcccgca cagatccagc aactcagatg 900 gaaaaggact gctgtcgctg ccgtagacct cgatgaactc caccccggcc gcgatgctgt 960 gcatgagggg ctcgacgtcc tcgatcaacg ttgtctttat gttggatcgc gacggcttgg 1020 tgacatcgat gatccgctgc accgcgggat cggacggatt tgcgatggtg tccaactcag 1080 tcatggtcgt cctaccggct gctgtgttca gtgacgcgat tcctggggtg tgacacccta 1140 cgcgacgatg gcggatggct gccctgaccg gcaatcacca acgcaagggg aagtcgtcgc 1200 tctctggcaa agctccccgc tcttccccgt ccgggacccg cgcggtcgat ccccgcatat 1260 atgaagtatt cgccttgatc agtcccggtg gacgcgccag cggcccgccg gagcgacgga 1320 ctccccgacc tcgatcgtgt cgccctgagc gtccacgtag acgttgcgtg agagcaggac 1380 tgggccgccg ccgaccgcac cgccctcacc accgaccgcg accgcgccat ggccgccgcc 1440 gacggcctgg tcgccgccgc cgcccgccgg ttcggcgcct gacccgacca acccccgcgg 1500 ggcgccggca cttcgtgctg gcgccccgcc cccacccacc aggagaccga ccatgaccga 1560 cttcgacgga cgcctgaccg aggggaccgt gaacctggtc caggacccca acggcggtgg 1620 ctggtccgcc cactgcgctg agcccggttg cgactgggcc gacttcgccg gaccgctcgg 1680 cttccagggc ctcgtggcca tcgctcgccg acacacgcac tgaccgcacg tcaaagcccc 1740 gccggatcac cggcggggct ctcttcggcc ctccaagtca caccagcccc aaggggcgtc 1800 gggagtggcg gagggaacct ctggcccgat tggtgccagg attcccacca gaccaaagag 1860 caacgggccg gacttcgcac ctccgacccg tccgctccca gactcgcgcc ccttagccgg 1920 gcgagacagg aacgttgctc gtgcccagag tacggagcga tgccgaggca ttgccagatc 1980 ggcccgccgg gccccgctgc cactgcggga ccgcaattgc ccacacaccg ggcaaacggc 2040 cgcgtatcta ctgctcagac cgctgccgga tggcagcgaa gcgggcgatc gcgcgtgtga 2100 cgcgagatgc cgcccgaggc aaaagcgaac accttgggaa agaaacaaca gagtttcccg 2160 cacccctccg acctgcggtt tctccggacg gggtggatgg ggagagcccg agaggcgaca 2220 gcctctcgga agtaggaagc acgtcgcgga gcgacgctgc ccgactgcgg aaagccgccc 2280 ggtacagccg ccgccggacg ctgtggcgga tcagcgggga cgccgcgtgc aagggctgcg 2340 gccgcgccct gatggaccct gcctccggcg taatcgtcgc ccagacggcg gccggaacgt 2400 ccgtggtcct gggcctgatg cggtgcgggc ggatctggct ctgcccggtc tgcgccgcca 2460 cgatccggca caagcgggcc gaggagatca ccgccgccgt ggtcgagtgg atcaagcgcg 2520 gggggaccgc ctacctggtc accttcacgg cccgccatgg gcacacggac cggctcgcgg 2580 acctcatgga cgccctccag ggcacccgga agacgccgga cagcccccgg cggccgggcg 2640 cctaccagcg actgatcacg ggcggcacgt gggccggacg ccgggccaag gacgggcacc 2700 gggccgccga ccgcgagggc atccgagacc ggatcgggta cgtcggcatg atccgcgcga 2760 ccgaagtcac cgtggggcag atcaacggct ggcacccgca catccacgcg atcgtcctgg 2820 tcggcggccg gaccgagggg gagcggtccg cgaagcagat cgtcgccacc ttcgagccga 2880 ccggcgccgc gctcgacgag tggcaggggc actggcggtc cgtgtggacc gccgccctgc 2940 gcaaggtcaa ccccgccttc acgcccgacg accggcacgg cgtcgacttc aagcggctgg 3000 agaccgagcg cgacgccaac gacctcgccg agtacatcgc caagacccag gacgggaagg 3060 cgcccgccct cgaactcgcc cgcgccgacc tcaagacggc gaccggcggg aacgtcgccc 3120 cgttcgaact cctcggacgg atcggggacc tgaccggcgg catgaccgag gacgacgccg 3180 ccggggtcgg ctcgctggag tggaacctct cgcgctggca cgagtacgag cgggcaaccc 3240 ggggacgccg ggccatcgaa tggacccgct acctgcggca gatgctcggg ctcgacggcg 3300 gcgacaccga ggccgacgac ctcgatctgc tcctggcggc cgacgccgac ggcggggagc 3360 tgcgggccgg ggtcgccgtg accgaggacg gatggcacgc ggtcacccgc cgcgccctcg 3420 acctcgaggc gacccgggcc gccgaaggca aggacggcaa cgaggattcg gcggccgtgg 3480 gcgaacgggt gcgggaggtc ctggcgctgg ccgacgcggc cgacacagtg gtggtgctca 3540 cggcggggga ggtggccgag gcgtacgccg acatgctcgc cgccctcgcc cagcgccgcg 3600 aggaagcaac tgcacgccga cggcgagagc aggacgacga ccaggacgac gacgccgacg 3660 accgccagga gcgggccgcc cggcacatcg cccggctcgc aagtgggccc acttcgcact 3720 aactcgctcc cccccgccgt acgtcatccc ggtgacgtac ggcgggggtc ggtgacgtac 3780 gcggcgacgg cggccggggt cgaagccgcg ggagtaatcc tgggattact cgcccggggt 3840 cggccccgcc ggcacttcgt gcaggcggta cctcgcgccc gactcgcctc gctacgagac 3900 gtgccgcgta cggtcgtcgg ccatgagcac caccaccccc agggacgccg acggcgcgaa 3960 gctctgcgcc tggtgcggct cggagatcaa gcaatccggc gtcggccgga gccgggacta 4020 ctgccgccgc tcctgccgcc agcgggcgta cgaggcccgg cgccagcgcg aggcgatcgt 4080 gtccgccgtg gcgtcggcag tcgctcgccg agatacgtca cgtgacgaaa tgcagcagcc 4140 ttccattccg tcacgtgacg aaactcgggc cgcaggtcag agcacggttc cgcccgctcc 4200 ggccctgccg gacccccggc tgcagctcgc ccggccgccg gtccccctgc cgtccggccc 4260 gtcccagagg cagcgtcggc ggctcctgcc tcccccgccc ggcgccgacc gggacccgca 4320 aaccccttga tccgctgtcg ggggtgatca cgtcagtttt cgcacgtgag ccacgccacc 4380 ggcggggcgc acccgcgcca aagaccaccg gaagggacgt ccgctcggaa aggaattgcc 4440 ccttccgccc gtcggcgagg accgccgcga gcaagatcat ctttgttcaa cattgcacga 4500 cagatcatta attgtccgga tcgcggccaa ccggtccggg ccgatttctc cccttctcct 4560 ccggtcgata ggtatgcggg gtcgtcaacc caacgcaccc caggaggtcc cgcatgccgg 4620 aactcacccg tcgtcgcgcg ctcggcgccg cagccgtcgt cgccgccggt gtcccgctgg 4680 tcgcccttcc cgccgcccgc gcggacgatc gggggcacca cacccccgag gtccccggga 4740 acccggccgc gtccggcgcc cccgccgcct tcgacgagat cccgcaaaag cggcctttga 4800 ctccctgcaa gcctcagcga ccgaatatat cggttatgcg tgggcgatgg ttgttgtcat 4860 tgtcggcgca actatcggta tcaagctgtt taagaaattc acctcgaaag caagctgata 4920 aaccgataca attaaaggct ccttttggag cctttttttt tggagatttt caacgtgaaa 4980 aaattattat tcgcaattcc tttagttgtt cctttctatt ctcactccgc tgaaactgtt 5040 gaaagttgtt tagcaaaacc tcatacagaa aattcattta ctaacgtctg gaaagacgac 5100 aaaactttag atctggggaa ttcgagctcg gtacccgggg atcccatatg gctgccgcgc 5160 ggcaccaggc cgctgctgtg atgatgatga tgatggctgc tgcccatgta tgtccgctcc 5220 cttctctgac gccgtccacg ctgcctcctc acgtgacgtg aggtgcaagc ccggacgttc 5280 cgcgtgccac gccgtgagcc gccgcgtgcc gtcggctccc tcagcccaga tccccgatcc 5340 ggtccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg 5400 gcgtttttta ttggtgagaa tcgcagcaac ttgtcgcgcc aatcgagcca tgtcgtcgtc 5460 aacgaccccc cattcaagaa cagcaagcag cattgagaac tttggaatcc agtccctctt 5520 ccacctgctg accggatcag cagtccccgg aacatcgtag ctgacgcctt cgcgttgctc 5580 agttgtccaa ccccggaaac gggaaaaagc aagttttccc cgctcacggc gtttcaataa 5640 ctgaaaacca tactatttca cagtttaaat cacattaaac gacagtaatc cccgttgatt 5700 tgtgcgccaa cacagatccc cgggtaccga gctgcattaa tgaatcggcc aacgcgcggg 5760 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 5820 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 5880 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 5940 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 6000 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 6060 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 6120 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 6180 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 6240 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 6300 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 6360 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 6420 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 6480 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 6540 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 6600 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 6660 ccttttggtt catgtgcagc tccatcagca aaaggggatg ataagtttat caccaccgac 6720 tatttgcaac agtgccgttg atcgtgctat gatcgactga tgtcatcagc ggtggagtgc 6780 aatgtcgtgc aatacgaatg gcgaaaagcc gagctcatcg gtcagcttct caaccttggg 6840 gttacccccg gcggtgtgct gctggtccac agctccttcc gtagcgtccg gcccctcgaa 6900 gatgggccac ttggactgat cgaggccctg cgtgctgcgc tgggtccggg agggacgctc 6960 gtcatgccct cgtggtcagg tctggacgac gagccgttcg atcctgccac gtcgcccgtt 7020 acaccggacc ttggagttgt ctctgacaca ttctggcgcc tgccaaatgt aaagcgcagc 7080 gcccatccat ttgcctttgc ggcagcgggg ccacaggcag agcagatcat ctctgatcca 7140 ttgcccctgc cacctcactc gcctgcaagc ccggtcgccc gtgtccatga actcgatggg 7200 caggtacttc tcctcggcgt gggacacgat gccaacacga cgctgcatct tgccgagttg 7260 atggcaaagg ttccctatgg ggtgccgaga cactgcacca ttcttcagga tggcaagttg 7320 gtacgcgtcg attatctcga gaatgaccac tgctgtgagc gctttgcctt ggcggacagg 7380 tggctcaagg agaagagcct tcagaaggaa ggtccagtcg gtcatgcctt tgctcggttg 7440 atccgctccc gcgacattgt ggcgacagcc ctgggtcaac tgggccgaga tccgttgatc 7500 ttcctgcatc cgccagaggc gggatgcgaa gaatgcgatg ccgctcgcca gtcgattggc 7560 tgagctcatg agcggagaac gagatgacgt tggaggggca aggtcgcgct gattgctggg 7620 gcaacacgtg gagcggatcg gggattgtct ttcttcagct cgctgatgat atgctgacgc 7680 tcaatgccgt ttggcctccg actaacgaaa atcccgcatt tggacggctg atccgattgg 7740 cacggcggac ggcgaatggc ggagcagacg ctcgtccggg ggcaatgaga tatgaaaaag 7800 cctgaactca ccgcgacgta tcgggccctg gccagctagc tagagtcgac ctgcaggtcc 7860 ccggggatcg gtcttgcctt gctcgtcggt gatgtacttc accagctccg cgaagtcgct 7920 cttcttgatg gagcgcatgg ggacgtgctt ggcaatcacg cgcacccccc ggccgtttta 7980 gcggctaaaa aagtcatggc tctgccctcg ggcggaccac gcccatcatg accttgccaa 8040 gctcgtcctg cttctcttcg atcttcgcca gcagggcgag gatcgtggca tcaccgaacc 8100 gcgccgtgcg cgggtcgtcg gtgagccaga gtttcagcag gccgcccagg cggcccaggt 8160 cgccattgat gcgggccagc tcgcggacgt gctcatagtc cacgacgccc gtgattttgt 8220 agccctggcc gacggccagc aggtaggccg acaggctcat gccggccgcc gccgcctttt 8280 cctcaatcgc tcttcgttcg tctggaaggc agtacacctt gataggtggg ctgcccttcc 8340 tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg gcggtagccg 8400 gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa gggacagtga 8460 agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc tgacgccgtt 8520 ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa 8580 aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat gcagcggaaa 8640 agatccgtcg acctgcaggc atg 8663 //