LOCUS New_DNA 3287 bp ds-DNA linear 07-DEC-2008 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind 24..41 /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind complement(2036..2056) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_recomb 57..156 /label=AttL1 /ApEinfo_fwdcolor=plum /ApEinfo_revcolor=plum misc_feature 1136..1141 /label=BamHI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 2128..2133 /label=XbaI /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=#00ffff misc_feature 226..231 /label=EcoRI /ApEinfo_fwdcolor=#0080ff /ApEinfo_revcolor=#0080ff misc_feature 1007..1135 /label=dblTerm /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 241..294 /label=P_tet of R0040 /ApEinfo_fwdcolor=#dfd0df /ApEinfo_revcolor=#dfd0df misc_feature 235..240 /label=BglII /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#00ff80 misc_feature 3281..3286 /label=XhoI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 320..997 /label=mRFP /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=#ff0080 misc_feature complement(1214..1233) /label=G00101 /ApEinfo_fwdcolor=#99e4fb /ApEinfo_revcolor=#99e4fb misc_feature 165..186 /label=CA998 /ApEinfo_fwdcolor=#9bd1f9 /ApEinfo_revcolor=#9bd1f9 misc_feature 1900..1999 /label=attL2 /ApEinfo_fwdcolor=#e9b4fa /ApEinfo_revcolor=#e9b4fa misc_feature 2268..3278 /label=specR /ApEinfo_fwdcolor=#f2f09d /ApEinfo_revcolor=#f2f09d misc_feature complement(2016..2035) /label=T7 Universal /ApEinfo_fwdcolor=#b19943 /ApEinfo_revcolor=#b19943 misc_feature complement(1240..1885) /label=oriV /ApEinfo_fwdcolor=#8ad2f4 /ApEinfo_revcolor=#8ad2f4 ORIGIN 1 CTAGTgtgga taaccgtcct aggtgTAAAA CGACGGCCAG TCTTAAGCTC GGGCCCCAAA 61 TAATGATTTT ATTTTGACTG ATAGTGACCT GTTCGTTGCA ACAAATTGAT GAGCAATGCT 121 TTTTTATAAT GCCAACTTTG TACAAAAAAG CAGGCTCCGA ATTGgtatca cgaggcagaa 181 tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctgGAATT CATGAGATCT 241 TCCCTATCAG TGATAGAGAT TGACATCCCT ATCAGTGATA GAGATACTGA GCACGGATCT 301 GAAAGAGGAG AAAGGATCTA TGGCAAGTAG CGAAGACGTT ATCAAAGAGT TCATGCGTTT 361 CAAAGTTCGT ATGGAAGGTT CCGTTAACGG TCACGAGTTC GAAATCGAAG GTGAAGGTGA 421 AGGTCGTCCG TACGAAGGTA CCCAGACCGC TAAACTGAAA GTTACCAAAG GTGGTCCGCT 481 GCCGTTCGCT TGGGACATCC TGTCCCCGCA GTTCCAGTAC GGTTCCAAAG CTTACGTTAA 541 ACACCCGGCT GACATCCCGG ACTACCTGAA ACTGTCCTTC CCGGAAGGTT TCAAATGGGA 601 ACGTGTTATG AACTTCGAAG ACGGTGGTGT TGTTACCGTT ACCCAGGACT CCTCCCTGCA 661 AGACGGTGAG TTCATCTACA AAGTTAAACT GCGTGGTACC AACTTCCCGT CCGACGGTCC 721 GGTTATGCAG AAAAAAACCA TGGGTTGGGA AGCTTCCACC GAACGTATGT ACCCGGAAGA 781 CGGTGCTCTG AAAGGTGAAA TCAAAATGCG TCTGAAACTG AAAGACGGTG GTCACTACGA 841 CGCTGAAGTT AAAACCACCT ACATGGCTAA AAAACCGGTT CAGCTGCCGG GTGCTTACAA 901 AACCGACATC AAACTGGACA TCACCTCCCA CAACGAAGAC TACACCATCG TTGAACAGTA 961 CGAACGTGCT GAAGGTCGTC ACTCCACCGG TGCTTAATAA GGATCTCCAG GCATCAAATA 1021 AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC 1081 GCTCTCTACT AGAGTCACAC TGGCTCACCT TCGGGTGGGC CTTTCTGCGT TTATAGGATC 1141 CtaaCTCGAc gtgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 1201 gcgagcggta tcagctcact caaaggcggt aatCAATTCt gcttcctcgc tcactcgacc 1261 gggagggttc gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg 1321 cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt 1381 tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac 1441 agcccctcaa atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc 1501 aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca 1561 cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg 1621 ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct 1681 gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca 1741 gtgagggcca agttttccgc gaggtatcca caacgccggc ggccggccgc ggtgtctcgc 1801 acacggcttc gacggcgttt ctggcgcgtt tgcagggcca tagacggccg ccagcccagc 1861 ggcgagggca accagccgag ggcttACGCG CCcaattcga cccagctttc ttgTACAAAG 1921 TTGGCATTAT AAAAAATAAT TGCTCATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC 1981 AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG 2041 TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC 2101 AAGATAAAAA TATATCATCA TGCCTCCTCT AGACCAGCCA GGACAGAAAT GCCTCGACTT 2161 CGCTGCTGCC CAAGGTTGCC GGGTGACGCA CACCGTGGAA ACGGATGAAG GCACGAACCC 2221 AGTGGACATA AGCCTGTTCG GTTCGTAAGC TGTAATGCAA GTAGCGTATG CGCTCACGCA 2281 ACTGGTCCAG AACCTTGACC GAACGCAGCG GTGGTAACGG CGCAGTGGCG GTTTTCATGG 2341 CTTGTTATGA CTGTTTTTTT GGGGTACAGT CTATGCCTCG GGCATCCAAG CAGCAAGCGC 2401 GTTACGCCGT GGGTCGATGT TTGATGTTAT GGAGCAGCAA CGATGTTACG CAGCAGGGCA 2461 GTCGCCCTAA AACAAAGTTA AACATCATGA GGGAAGCGGT GATCGCCGAA GTATCGACTC 2521 AACTATCAGA GGTAGTTGGC GTCATCGAGC GCCATCTCGA ACCGACGTTG CTGGCCGTAC 2581 ATTTGTACGG CTCCGCAGTG GATGGCGGCC TGAAGCCACA CAGTGATATT GATTTGCTGG 2641 TTACGGTGAC CGTAAGGCTT GATGAAACAA CGCGGCGAGC TTTGATCAAC GACCTTTTGG 2701 AAACTTCGGC TTCCCCTGGA GAGAGCGAGA TTCTCCGCGC TGTAGAAGTC ACCATTGTTG 2761 TGCACGACGA CATCATTCCG TGGCGTTATC CAGCTAAGCG CGAACTGCAA TTTGGAGAAT 2821 GGCAGCGCAA TGACATTCTT GCAGGTATCT TCGAGCCAGC CACGATCGAC ATTGATCTGG 2881 CTATCTTGCT GACAAAAGCA AGAGAACATA GCGTTGCCTT GGTAGGTCCA GCGGCGGAGG 2941 AACTCTTTGA TCCGGTTCCT GAACAGGATC TATTTGAGGC GCTAAATGAA ACCTTAACGC 3001 TATGGAACTC GCCGCCCGAC TGGGCTGGCG ATGAGCGAAA TGTAGTGCTT ACGTTGTCCC 3061 GCATTTGGTA CAGCGCAGTA ACCGGCAAAA TCGCGCCGAA GGATGTCGCT GCCGACTGGG 3121 CAATGGAGCG CCTGCCGGCC CAGTATCAGC CCGTCATACT TGAAGCTAGA CAGGCTTATC 3181 TTGGACAAGA AGAAGATCGC TTGGCCTCGC GCGCAGATCA GTTGGAAGAA TTTGTCCACT 3241 ACGTGAAAGG CGAGATCACC AAGGTAGTCG GCAAATAACC CTCGAGG //