LOCUS                 pBca1256        2982 bp ds-DNA   linear       31-JAN-2009
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     44..61
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     primer_bind     complement(997..1017)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_recomb     77..176
                     /label=AttL1
                     /ApEinfo_fwdcolor=plum
                     /ApEinfo_revcolor=plum
     misc_feature    756..761
                     /label=BamHI
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
     misc_feature    1089..1094
                     /label=XbaI
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#00ffff
     misc_feature    246..251
                     /label=EcoRI
                     /ApEinfo_fwdcolor=#0080ff
                     /ApEinfo_revcolor=#0080ff
     misc_feature    255..260
                     /label=BglII
                     /ApEinfo_fwdcolor=#00ff80
                     /ApEinfo_revcolor=#00ff80
     misc_feature    2242..2247
                     /label=XhoI
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
     misc_feature    complement(834..853)
                     /label=G00101
                     /ApEinfo_fwdcolor=#99e4fb
                     /ApEinfo_revcolor=#99e4fb
     misc_feature    185..206
                     /label=CA998
                     /ApEinfo_fwdcolor=#9bd1f9
                     /ApEinfo_revcolor=#9bd1f9
     misc_feature    861..960
                     /label=attL2
                     /ApEinfo_fwdcolor=#e9b4fa
                     /ApEinfo_revcolor=#e9b4fa
     misc_feature    1229..2239
                     /label=specR
                     /ApEinfo_fwdcolor=#f2f09d
                     /ApEinfo_revcolor=#f2f09d
     misc_feature    2253..2919
                     /label=colE1
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#c0c0c0
     misc_feature    261..755
                     /label=T4 Lysozyme
                     /ApEinfo_fwdcolor=#ff0080
                     /ApEinfo_revcolor=#ff0080
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTcct aggTGTAAAA CGACGGCCAG
       61 TCTTAAGCTC GGGCCCCAAA TAATGATTTT ATTTTGACTG ATAGTGACCT GTTCGTTGCA
      121 ACAAATTGAT GAGCAATGCT TTTTTATAAT GCCAACTTTG TACAAAAAAG CAGGCTCCGA
      181 ATTGgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat
      241 ttctgGAATT CATGAGATCT atgaatatat ttgaaatgtt acgtatagat gaaggtctta
      301 gacttaaaat ctataaagac acagaaggct attacactat tggcatcggt catttgctta
      361 caaaaagtcc atcacttaat gctgctaaat ctgaattaga taaagctatt gggcgtaatt
      421 gcaatggtgt aattacaaaa gatgaggctg aaaaactctt taatcaggat gttgatgctg
      481 ctgttcgcgg aatcctgaga aatgctaaat taaaaccggt ttatgattct cttgatgcgg
      541 ttcgtcgctg tgcattgatt aatatggttt tccaaatggg agaaaccggt gtggcaggat
      601 ttactaactc tttacgtatg cttcaacaaa aacgctggga tgaagcagca gttaacttag
      661 ctaaaagtag atggtataat caaacaccta atcgcgcaaa acgagtcatt acaacgttta
      721 gaactggcac ttgggacgcg tataaaaatc tataaGGATC CtaaCTCGAc gtgcaggctt
      781 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      841 caaaggcggt aatCAATTCG ACCCAGCTTT CTTGTACAAA GTTGGCATTA TAAAAAATAA
      901 TTGCTCATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG
      961 CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA
     1021 GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA ATATATCATC
     1081 ATGCCTCCTC TAGACCAGCC AGGACAGAAA TGCCTCGACT TCGCTGCTGC CCAAGGTTGC
     1141 CGGGTGACGC ACACCGTGGA AACGGATGAA GGCACGAACC CAGTGGACAT AAGCCTGTTC
     1201 GGTTCGTAAG CTGTAATGCA AGTAGCGTAT GCGCTCACGC AACTGGTCCA GAACCTTGAC
     1261 CGAACGCAGC GGTGGTAACG GCGCAGTGGC GGTTTTCATG GCTTGTTATG ACTGTTTTTT
     1321 TGGGGTACAG TCTATGCCTC GGGCATCCAA GCAGCAAGCG CGTTACGCCG TGGGTCGATG
     1381 TTTGATGTTA TGGAGCAGCA ACGATGTTAC GCAGCAGGGC AGTCGCCCTA AAACAAAGTT
     1441 AAACATCATG AGGGAAGCGG TGATCGCCGA AGTATCGACT CAACTATCAG AGGTAGTTGG
     1501 CGTCATCGAG CGCCATCTCG AACCGACGTT GCTGGCCGTA CATTTGTACG GCTCCGCAGT
     1561 GGATGGCGGC CTGAAGCCAC ACAGTGATAT TGATTTGCTG GTTACGGTGA CCGTAAGGCT
     1621 TGATGAAACA ACGCGGCGAG CTTTGATCAA CGACCTTTTG GAAACTTCGG CTTCCCCTGG
     1681 AGAGAGCGAG ATTCTCCGCG CTGTAGAAGT CACCATTGTT GTGCACGACG ACATCATTCC
     1741 GTGGCGTTAT CCAGCTAAGC GCGAACTGCA ATTTGGAGAA TGGCAGCGCA ATGACATTCT
     1801 TGCAGGTATC TTCGAGCCAG CCACGATCGA CATTGATCTG GCTATCTTGC TGACAAAAGC
     1861 AAGAGAACAT AGCGTTGCCT TGGTAGGTCC AGCGGCGGAG GAACTCTTTG ATCCGGTTCC
     1921 TGAACAGGAT CTATTTGAGG CGCTAAATGA AACCTTAACG CTATGGAACT CGCCGCCCGA
     1981 CTGGGCTGGC GATGAGCGAA ATGTAGTGCT TACGTTGTCC CGCATTTGGT ACAGCGCAGT
     2041 AACCGGCAAA ATCGCGCCGA AGGATGTCGC TGCCGACTGG GCAATGGAGC GCCTGCCGGC
     2101 CCAGTATCAG CCCGTCATAC TTGAAGCTAG ACAGGCTTAT CTTGGACAAG AAGAAGATCG
     2161 CTTGGCCTCG CGCGCAGATC AGTTGGAAGA ATTTGTCCAC TACGTGAAAG GCGAGATCAC
     2221 CAAGGTAGTC GGCAAATAAC CCTCGAGCCA CCCATGACCA AAATCCCTTA ACGTGAGTTA
     2281 CGCGTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC
     2341 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT
     2401 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC
     2461 GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC
     2521 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG
     2581 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG
     2641 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA
     2701 ACTGAGATAC CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC
     2761 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG
     2821 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG
     2881 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT
     2941 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TT 
//