LOCUS New_DNA 4081 bp ds-DNA circular 01-FEB-2009 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT Insert from C:/Users/JCAnderson/Documents/Sequences/pBca1256.ape 160 to 1270 Backbone from C:/Users/JCAnderson/Documents/Sequences/pBca1254AK.ape 1003 to 23 COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_recomb 1..17 /label=AttL1 /ApEinfo_fwdcolor=plum /ApEinfo_revcolor=plum CDS 3114..3974 /label=AmpR /ApEinfo_fwdcolor=#f8c887 /ApEinfo_revcolor=#f8c887 misc_feature 2932..2937 /label=PstI /ApEinfo_fwdcolor=#0080ff /ApEinfo_revcolor=#0080ff misc_feature 997..1002 /label=BamHI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_recomb 2938..2971 /label=LoxP /ApEinfo_fwdcolor=steel blue /ApEinfo_revcolor=orange misc_feature 2972..2977 /label=XhoI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 misc_feature 87..92 /label=EcoRI /ApEinfo_fwdcolor=#0080ff /ApEinfo_revcolor=#0080ff misc_recomb 4074..4081 /label=AttR1 /ApEinfo_fwdcolor=plum /ApEinfo_revcolor=plum misc_feature 868..996 /label=dblTerm /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_recomb complement(1112..1126) /label=AttR2 /ApEinfo_fwdcolor=plum /ApEinfo_revcolor=plum misc_feature 102..155 /label=P_tet of R0040 /ApEinfo_fwdcolor=#dfd0df /ApEinfo_revcolor=#dfd0df misc_feature 96..101 /label=BglII /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#00ff80 misc_feature complement(1968..2783) /label=KnR Kanamycin Resistance /ApEinfo_fwdcolor=#548d5c /ApEinfo_revcolor=#548d5c misc_feature 181..858 /label=mRFP /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=#ff0080 misc_feature 1223..1905 /label=colE1 ori /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=#c0c0c0 misc_feature complement(1075..1094) /label=G00101 /ApEinfo_fwdcolor=#99e4fb /ApEinfo_revcolor=#99e4fb misc_feature 26..47 /label=CA998 /ApEinfo_fwdcolor=#9bd1f9 /ApEinfo_revcolor=#9bd1f9 misc_feature 1102..1111 /label=attL2 /ApEinfo_fwdcolor=#e9b4fa /ApEinfo_revcolor=#e9b4fa ORIGIN 1 GTACAAAAAA GCAGGCTCCG AATTGgtatc acgaggcaga atttcagata aaaaaaatcc 61 ttagctttcg ctaaggatga tttctgGAAT TCATGAGATC TTCCCTATCA GTGATAGAGA 121 TTGACATCCC TATCAGTGAT AGAGATACTG AGCACGGATC TGAAAGAGGA GAAAGGATCT 181 ATGGCAAGTA GCGAAGACGT TATCAAAGAG TTCATGCGTT TCAAAGTTCG TATGGAAGGT 241 TCCGTTAACG GTCACGAGTT CGAAATCGAA GGTGAAGGTG AAGGTCGTCC GTACGAAGGT 301 ACCCAGACCG CTAAACTGAA AGTTACCAAA GGTGGTCCGC TGCCGTTCGC TTGGGACATC 361 CTGTCCCCGC AGTTCCAGTA CGGTTCCAAA GCTTACGTTA AACACCCGGC TGACATCCCG 421 GACTACCTGA AACTGTCCTT CCCGGAAGGT TTCAAATGGG AACGTGTTAT GAACTTCGAA 481 GACGGTGGTG TTGTTACCGT TACCCAGGAC TCCTCCCTGC AAGACGGTGA GTTCATCTAC 541 AAAGTTAAAC TGCGTGGTAC CAACTTCCCG TCCGACGGTC CGGTTATGCA GAAAAAAACC 601 ATGGGTTGGG AAGCTTCCAC CGAACGTATG TACCCGGAAG ACGGTGCTCT GAAAGGTGAA 661 ATCAAAATGC GTCTGAAACT GAAAGACGGT GGTCACTACG ACGCTGAAGT TAAAACCACC 721 TACATGGCTA AAAAACCGGT TCAGCTGCCG GGTGCTTACA AAACCGACAT CAAACTGGAC 781 ATCACCTCCC ACAACGAAGA CTACACCATC GTTGAACAGT ACGAACGTGC TGAAGGTCGT 841 CACTCCACCG GTGCTTAATA AGGATCTCCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA 901 AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCTAC TAGAGTCACA 961 CTGGCTCACC TTCGGGTGGG CCTTTCTGCG TTTATAGGAT CCtaaCTCGA cgtgcaggct 1021 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 1081 tcaaaggcgg taatCAATTC GACCCAGCTT TCTTGTACAA AGTGGTTGAT CCTTACAGAT 1141 CCcggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 1201 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc 1261 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 1321 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 1381 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 1441 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 1501 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 1561 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 1621 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 1681 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 1741 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 1801 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 1861 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgaACTAG Ctcccgtcaa 1921 gtcagcgtaa tgctctgcca gtgttacaac caattaacca attctgatta gaaaaactca 1981 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga 2041 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga 2101 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc 2161 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag 2221 aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg 2281 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga 2341 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc 2401 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc 2461 tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg 2521 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc 2581 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca 2641 tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc 2701 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct ggagcaagac 2761 gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt 2821 tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac 2881 acaacgtggc tttgttgaat aaatcgaact tttgctgagt tgaaggatca gCTGCAGata 2941 acttcgtata gcatacatta tacgaagtta tctcgaggac gtcaggtggc acttttcggg 3001 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 3061 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 3121 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 3181 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 3241 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 3301 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 3361 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 3421 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 3481 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 3541 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 3601 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 3661 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 3721 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 3781 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 3841 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 3901 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 3961 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 4021 ttcattttta atttaaaaGG ATCTAGGTGA AGATCCTTGa attgCCATTA TCAACAAGTT 4081 T //