LOCUS pBgl00001_Bca185 2618 bp ds-DNA linear 06-FEB-2012 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind 2417..2434 /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(1140..1160) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 2450..2549 /label=AttL1 /ApEinfo_fwdcolor=plum /ApEinfo_revcolor=plum /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(1550..2390) /label=p15A origin /ApEinfo_fwdcolor=#956a92 /ApEinfo_revcolor=#956a92 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1304..1540 /label=DHFR /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(977..996) /label=G00101 /ApEinfo_fwdcolor=#99e4fb /ApEinfo_revcolor=#99e4fb /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2558..2579 /label=CA998 /ApEinfo_fwdcolor=#9bd1f9 /ApEinfo_revcolor=#9bd1f9 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1004..1103 /label=attL2 /ApEinfo_fwdcolor=#e9b4fa /ApEinfo_revcolor=#e9b4fa /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(1120..1139) /label=T7 Universal /ApEinfo_fwdcolor=#b19943 /ApEinfo_revcolor=#b19943 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10..15 /label=BglII /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#00ff80 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 899..904 /label=BamHI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1..6 /label=EcoRI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1541..1546 /label=XhoI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1232..1237 /label=XbaI /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=#00ffff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(311..316,331..332) /label=AcuI/Eco57I /ApEinfo_fwdcolor=#d2d068 /ApEinfo_revcolor=#d2d068 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(1458..1463,1478..1479) /label=AcuI/Eco57I(1) /ApEinfo_label=AcuI/Eco57I /ApEinfo_fwdcolor=#d2d068 /ApEinfo_revcolor=#d2d068 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(167..172,181..182) /label=BseRI /ApEinfo_fwdcolor=#9ac688 /ApEinfo_revcolor=#9ac688 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(1701..1706,1715..1716) /label=BseRI(1) /ApEinfo_label=BseRI /ApEinfo_fwdcolor=#9ac688 /ApEinfo_revcolor=#9ac688 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(join(902..903,912..917)) /label=BseRI(2) /ApEinfo_label=BseRI /ApEinfo_fwdcolor=#9ac688 /ApEinfo_revcolor=#9ac688 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(join(1218..1219,1228..1233)) /label=BseRI(3) /ApEinfo_label=BseRI /ApEinfo_fwdcolor=#9ac688 /ApEinfo_revcolor=#9ac688 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(join(1758..1759,1768..1773)) /label=BseRI(4) /ApEinfo_label=BseRI /ApEinfo_fwdcolor=#9ac688 /ApEinfo_revcolor=#9ac688 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 182..898 /label=sfGFP /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 16..145 /label=PglpT /ApEinfo_fwdcolor=#800040 /ApEinfo_revcolor=#800040 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 gaattcatgA GATCTgaaag tgaaacgtga tttcatgcgt cattttgaac attttgtaaa 61 tcttatttaa taatgtgtgc ggcaattcac atttaattta tgaatgtttt cttaacatcg 121 cggcaactca agaaacggca ggttcGGATC Ttagctacta gagaaagagg agaaatacta 181 gatgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct attctggtgg aactggatgg 241 tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt gaaggtgacg caactaatgg 301 taaactgacg ctgaagttca tctgtactac tggtaaactg ccggtacctt ggccgactct 361 ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt tatccggacc atatgaagca 421 gcatgacttc ttcaagtccg ccatgccgga aggctatgtg caggaacgca cgatttcctt 481 taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa tttgaaggcg ataccctggt 541 aaaccgcatt gagctgaaag gcattgactt taaagaagac ggcaatatcc tgggccataa 601 gctggaatac aattttaaca gccacaatgt ttacatcacc gccgataaac aaaaaaatgg 661 cattaaagcg aattttaaaa ttcgccacaa cgtggaggat ggcagcgtgc agctggctga 721 tcactaccag caaaacactc caatcggtga tggtcctgtt ctgctgccag acaatcacta 781 tctgagcacg caaagcgttc tgtctaaaga tccgaacgag aaacgcgatc atatggttct 841 gctggagttc gtaaccgcag cgggcatcac gcatggtatg gatgaactgt acaaatgaGG 901 ATCCtaaCTC GCTCCTCagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 961 gcggcgagcg gtatcagctc actcaaaggc ggtaatCAAT TCGACCCAGC TTTCTTGTAC 1021 AAAGTTGGCA TTATAAAAAA TAATTGCTCA TCAATTTGTT GCAACGAACA GGTCACTATC 1081 AGTCAAAATA AAATCATTAT TTGCCATCCA GCTGATATCC CCTATAGTGA GTCGTATTAC 1141 ATGGTCATAG CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT 1201 GCACAAGATA AAAATATATC ATCATGCCTC CTCTAGAgtt gacaattaat catcggcata 1261 gtatatcggc atagtataat acgacaaggt gaggaactaa accATGGGTC AAAGTAGCGA 1321 TGAAGCCAAC GCTCCCGTTG CAGGGCAGTT TGCGCTTCCC CTGAGTGCCA CCTTTGGCTT 1381 AGGGGATCGC GTACGCAAGA AATCTGGTGC CGCTTGGCAG GGTCAAGTCG TCGGTTGGTA 1441 TTGCACAAAA CTCACTCCTG AAGGCTATGC GGTCGAGTCC GAATCCCACC CAGGCTCAGT 1501 GCAAATTTAT CCTGTGGCTG CACTTGAACG TGTGGCCTAA CTCGAGtaga aatattttat 1561 ctgattaata agatgatctt cttgagatcg ttttggtctg cgcgtaatct cttgctctga 1621 aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt ctctgagcta ccaactcttt 1681 gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa cttgtccttt cagtttagcc 1741 ttaaccggcg catgacttca agactaactc ctctaaatca attaccagtg gctgctgcca 1801 gtggtgcttt tgcatgtctt tccgggttgg actcaagacg atagttaccg gataaggcgc 1861 agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc 1921 cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg 1981 gtaaaccgaa aggcaggaac aggagagcgc acgagggagc cgccaggggg aaacgcctgg 2041 tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat ttcgtgatgc 2101 ttgtcagggg ggcggagcct atggaaaaac ggctttgccg cggccctctc acttccctgt 2161 taagtatctt cctggcatct tccaggaaat ctccgccccg ttcgtaagcc atttccgctc 2221 gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa tatatcctgt 2281 atcacatatt ctgctgacgc accggtgcag ccttttttct cctgccacat gaagcacttc 2341 actgacaccc tcatcagtgc caacatagta agccagtata cactccgcta gcgctgatgt 2401 ccggcggtgc cctaggTGTA AAACGACGGC CAGTCTTAAG CTCGGGCCCC AAATAATGAT 2461 TTTATTTTGA CTGATAGTGA CCTGTTCGTT GCAACAAATT GATGAGCAAT GCTTTTTTAT 2521 AATGCCAACT TTGTACAAAA AAGCAGGCTC CGAATTGgta tcacgaggca gaatttcaga 2581 taaaaaaaat CCTTAGCTTT CGCTAAGGAT CTGAAGTG //