View source for Protocols

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==Protocols in Development==

'''''Please Contribute!'''''
* [[Pulse-chase protein production]]
* [[Fixing cells]] 
* [[Real-time RT-PCR]]
* [[B-galactosidase assay|&beta;-galactosidase assay]]  
* [[Denaturing acrylamide gel purification of nucleic acids]] 
* [[Quantification of nucleic acids]] 
* [[Bacterial species identification]]
* [[Yeast artificial chromosomes]]
* [[One step 'miniprep' method for the isolation of plasmid DNA]]

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==''In vivo''==

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{{size|2|'''''Drosophila'''''}}__HIDER__
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* [[Drosophila CHiP on chip|Drosophila CHiP on Chip]] 
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{{size|2|'''''Escherichia coli'''''}}__HIDER__
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* [[Bacterial cell culture]] 
* [[Bacterial Transformation ]] 
** [[Preparing chemically competent cells]] 
** [[Transforming chemically competent cells]] 
** [[Electrocompetent cells]] 
** [[Electroporation]] 
** [[Preparing chemically competent cells (Inoue)]]
** [[Transforming chemically competent cells (Inoue)]]
* [[beta-Galactosidase Assay (A better Miller)]]
* [[Colony PCR]] 
* [[Making a long term stock of bacteria]] 
* [[Miniprep]] 
* [[P1vir phage transduction]] 
* [[Recombineering]]
* [[Endy:Pouring plates]] 
* [http://www.cf.ac.uk/biosi/staff/ehrmann/tools/factors.htm Protein expression resource] 
* [[S_N Chemostat Protocol]] 
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{{size|2|'''''Mesoplasma florum'''''}}__HIDER__
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* [[Mesoplama florum:Culture media|Culture media]] 
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{{size|2|'''Mouse'''}}__HIDER__
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* [[Mouse keratinocyte cultures]]
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{{size|2|'''T7'''}}__HIDER__
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* [[Cesium Chloride Purification of T7]] 
* [[Studier Lysate Prep]] 
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{{size|2|'''Yeast'''}}__HIDER__
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* [[Assaying mating]] 
* [[Cell Cycle Arrest]] 
* [[Dropout plates for yeast]] 
* [[High Efficiency Transformation]] 
* [[Layered plates]] 
* [[Pour YPD plates]] 
* [[Silver: Lysate for Western]] <font size=1>(for Western etc.)</font> 
* [[Silver: Time-Lapse Microscopy]] 
* [[Yeast Colony PCR]] 
* [[Yeast DNA Prep]] 
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<!-----------------------------------In vitro----------------------------------->
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==''In vitro''==
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{{size|2|'''Nucleic acids'''}}__HIDER__
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* [[Nucleic acid precipitation]] 
* [[Phenol/chloroform extraction]] 
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{{size|2|'''DNA'''}}__HIDER__
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* [[Agarose gel electrophoresis]] 
* [[Annealing Primers]] 
* [[Designing primers]] 
* [[DNA Ligation]] 
* [[Double stranding oligo libraries]] 
* [[Library Generation]] 
* [[Miniprep]] 
* [[PCR]] 
* [[Phosphatase treatment of linearized vector]] 
* [http://arep.med.harvard.edu/polony/polony_protocols Polony Protocols]
* [[PNK Treatment of DNA Ends]] 
* [[Purification of DNA]] 
* [[Reconstituting primers]] 
* [[Restriction digest]] 
* [['Round-the-horn site-directed mutagenesis]] 
* [[Sequencing DNA]] 
* [[Site-directed mutagenesis]] 
* [[Templiphi]] 
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{{size|2|'''RNA'''}}__HIDER__
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* [[In vitro transcription with T7 RNA polymerase]] 
* [[Northern Blot, 32P End-Labeled Probes ]] 
* [[Probe Prep, 32P End-Labeled Probes]] 
* [[RNA Extraction]] 
* [[RNase Protection Assay]] 
* [[RNA Half-life]]
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{{size|2|'''Protein'''}}__HIDER__
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* [[Acrylamide Gels]] 
* [[Silver: Destain]] 
* [[Silver: Coomassie Stain]] 
* [[Keating:Experimental Protocols:HPLC]] 
* [[Silver staining]] 
* [[Western Blot]] 
* [[Purification of His-tagged proteins]] 
* [[Free Sulfhydryl Determination]]
*[[Sauer:Expression/purification of 35S-Met proteins|Expression and purification of <sup>35</sup>S-labeled proteins]]
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<!-----------------------------------In silico------------------------------------>
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==''In silico''==
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{{size|2|'''Sequence Analysis'''}}__HIDER__
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* [[BLAST]] 
* [[CLUSTALW]] 
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{{size|2|'''Structure'''}}__HIDER__
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* [[THREADER]] 
* [[Homology Modelling]] 
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{{size|2|'''Pathway Modelling'''}}__HIDER__
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* [[FBA]] 
* [[MoMA]] 
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<!-----------------------------------OTHER--------------------------------------->
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==Other==
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{{size|2|'''Flow Cytometry'''}}__HIDER__
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* [[Flow Calibration]] 
* [[Endy:FACS protocols]] 
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{{size|2|'''Microfluidics'''}}__HIDER__
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* [[Design a Microfluidic Chip]] 
* [[Order a Microfluidic Chip from the Foundry]] 
* [[Operate Microfluidic Chemostat]] 
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{{size|2|'''Microscopy'''}}__HIDER__
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* [[Agarose Pads for Microscopy]] 
* [[Quantitative Microscopy]] 
* [[Silver: Time-Lapse Microscopy]] 
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{{size|2|'''Plate reader'''}}__HIDER__
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* [[Endy:Victor3 plate reader]]<font size=1>Plate reader usage protocols</font> 
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{{size|2|'''General resources'''}}__HIDER__
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* [http://www.mrw.interscience.wiley.com/cp/cpmb/cpmb_contents_fs.html Current Protocols in Molecular Biology] <font size=1>Downloadable pdfs.</font>
*[http://www.molecularcloning.com/members/toc.jsp Molecular Cloning] 
* [http://protocol-online.org Protocol-online] <font size=1>Useful protocols and a popular discussion section.</font> 
* [http://www.invitrogen.com/iProtocol iProtocol] <font size=1>Abridged ''Current Protocols'' Invitrogen protocols.  Not amenable to printing.</font>
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