View source for Yu:PBS

{{Template:Yu}}

{| border="0" cellpadding="10"
|+'''Solution recipe for 1L 10x <span id="PBS">Phosphate-Buffered Saline</span>. Dilute to 1x for use.'''
|width="600px" valign="top"|1. Measure 700mL millipore water into a 2L plastic beaker.
|width="100px" valign="top"|<u>10x</u>
|-
|2. Weigh 80g NaCl and add it to the mixture
|1.4M NaCl
|-
|5. Weigh 2g KCl and add it to the mixture
|30mM KCl
|-
|4. Weigh 14.4g Na<sub>2</sub>HPO<sub>4</sub> and add it to the mixture
|100mM Na<sub>2</sub>HPO<sub>4</sub>
|-
|5. Weigh 2.4g KH<sub>2</sub>PO<sub>4</sub> and add it to the mixture
|20mM KH<sub>2</sub>PO<sub>4</sub>
|-
|6. Stir until dissolved
|
|-
|7. Bring volume to 1L with millipore water in a graduated cylinder
|
|-
|8. Dilute from 10x to 1x (1:10) for use
|}


{| border="0" cellpadding="10"
|+'''Solution recipe for 1L 1x <span id="PBST">PBST</span>'''.
|width="600px" valign="top"|1. Measure 1L 1x PBS into a 1L bottle.
|width="100px" valign="top"|1L 1x [[Yu:PBS#PBS|PBS]]
|-
|2. Pipette 2.5mL [[Tween|TWEEN 20]] into the bottle
|0.25% [[Tween|TWEEN 20]]
|-
|3. Stir until mixed
|
|}


{| border="0" cellpadding="10"
|+'''Solution recipe for 1L 1x <span id="PBSMT">PBSMT</span>'''.
|width="600px" valign="top"|1. Measure 100mL 10x PBS into a beaker.
|width="100px" valign="top"|1x [[Yu:PBS#PBS|PBS]]
|-
|2. Add 3mL 1M KCl
|3mM KCl 
|-
|3. Add 2.5mL 1M MgCl<sub>2</sub>
|2.5mM MgCl<sub>2</sub>
|-
|4. Add 5mL [[Triton X-100]]
|0.5% [[Triton X-100]]
|-
|5. Bring to 1L volume with H<sub>2</sub>O and stir until mixed
|
|}

[[Category:Yu Solutions]]