View source for Yu:TBS

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|+'''Solution recipe for 1L 10x <span id="TBS">Tris-Buffered Saline</span>. Dilute to 1x for use.'''
|width="600px" valign="top"|1. Measure 700mL millipore water into a 2L plastic beaker.
|width="100px" valign="top"|<u>10x</u>
|-
|2. Weigh and add 87.7g NaCl
|1.5M NaCl
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|3. Add 100mL 1M TRIS-HCl pH 7.5
|100mM TRIS pH 7.5
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|4. Stir until dissolved
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|5. Bring volume to 1L with millipore water in a graduated cylinder
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|6. Dilute to 1x (1:10) for use
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{| border="0" cellpadding="10"
|+'''Solution recipe for 1L <span id="TBS-TT">Tris-Buffered Saline / Tween-Triton</span>'''
|width="600px" valign="top"|1. Make 1L of 1x TBS by diluting 10x stock with millipore H<sub>2</sub>O
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|-
|2. Add 2.5mL TWEEN-20
|0.25% TWEEN
|-
|3. Add 10mL Triton-X
|1% Triton-X
|-
|Vary detergents by intended application
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