User:Carly M. Montanero/Notebook/CHEM-571/2013/10/16: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Fall 2013</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Fall 2013</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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# Add 1.00 mL of 2.33 mM gold solution (HAuCL<sub>4</sub>) to a 10 mL volumetric flask. | # Add 1.00 mL of 2.33 mM gold solution (HAuCL<sub>4</sub>) to a 10 mL volumetric flask. | ||
# Add 1.73 mL BSA (90x more gold than BSA) to the volumetric flask. | # Add 1.73 mL BSA (90x more gold than BSA) to the volumetric flask. | ||
# Dilute | # Dilute the solution up to 10 mL with deionized water. | ||
# Transfer the solution to a test tube and cap with aluminum foil. | # Transfer the solution to a test tube and cap with aluminum foil. | ||
# Heat in the oven at 80°C for four hours. | # Heat in the oven at 80°C for four hours. | ||
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# The 10 mL solution of gold and HRP was placed into a test tube. | # The 10 mL solution of gold and HRP was placed into a test tube. | ||
# The test tube was placed in the oven at 80°C for four hours. | # The test tube was placed in the oven at 80°C for four hours. | ||
==Figures== | |||
[[Image:Cmj_HRPKineticsUV-Vis_10.16.2013.png ]] | |||
*Note: Spectra overlap for a total elapsed time of 19 minutes. | |||
[[Image:Cmj_HRPKinetics_520overtime_10.16.2013.png]] | |||
==Notes== | ==Notes== | ||
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* The stock concentration of gold (HAuCL<sub>4</sub>)was 2.33 mM. | * The stock concentration of gold (HAuCL<sub>4</sub>)was 2.33 mM. | ||
* The stock concentration of BSA was 15 μM. | * The stock concentration of BSA was 15 μM. | ||
* The stock solution of gold was 2.94 mM. | |||
* The stock solution of HRP was 16.27 μM. | |||
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Latest revision as of 23:29, 26 September 2017
Biomaterials Design Lab: Fall 2013 | Main project page Previous entry Next entry |
ObjectiveTo test the activity of our HRP-NPs for the catalytic conversion of luminol. ProcedureCreating the Luminol-H2O2 Solution
Enzyme Kinetics Measurement
BSA-AuNP Synthesis
Remaking the Solution of Gold and HRP
Figures
Notes
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