User:Desiree Carmen/Notebook/AU Biomaterial Lab/2014/04/11: Difference between revisions
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# Add 244μL of 0.55mg/mL protein to the test tube | # Add 244μL of 0.55mg/mL protein to the test tube | ||
# Add 250μL of 10mM NH<sub>4</sub>OH | # Add 250μL of 10mM NH<sub>4</sub>OH | ||
## This should bring the pH up to 8.2. Check on this with a pH strip ( | ## This should bring the pH up to 8.2. Check on this with a pH strip (290μL 10mM NH<sub>4</sub>OH) | ||
# Bring the volume up to 2.5mL with water (Add 1316μL of water) | # Bring the volume up to 2.5mL with water (Add 1316μL of water) | ||
# Incubate at room temperature for 1 hour | # Incubate at room temperature for 1 hour | ||
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## When you add this add it drop wise (as best as you can) while vigorously shaking the test tube. | ## When you add this add it drop wise (as best as you can) while vigorously shaking the test tube. | ||
# Incubate at 37C for 5 days. | # Incubate at 37C for 5 days. | ||
Repeat Steps using Syringe Method in another test tube | |||
Add materials as listed above; However, add in each reactant into sealed tube using 10mL syringe and hydrochrome needle. | |||
500μL of 10mM NH<sub>4</sub>OH was added | |||
1356μL of water added to bring volume up to 2.5mL. | |||
==Data== | ==Data== |
Revision as of 09:37, 11 April 2014
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ObjectiveSynthesize Mn-doped ZnS quantum dots using the synthesis described here DescriptionSynthesis Protocol for Mn-doped ZnS nanocrystals with an Mn:Zn ratio of 1:7.5 and a final (Zn+Mn) concentration of 0.4mM
Repeat Steps using Syringe Method in another test tube Add materials as listed above; However, add in each reactant into sealed tube using 10mL syringe and hydrochrome needle. 500μL of 10mM NH4OH was added 1356μL of water added to bring volume up to 2.5mL. DataStock Solutions All solutions are made in 250mL volumetric flasks.
NotesThis area is for any observations or conclusions that you would like to note.
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