BME494 Sp2014 Tran

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Background & Proposed Application The malarial biosensor is modeled after the classical synthetic toggle switch developed in Escherichia coli, or E. Coli (Gardner et al 2000). The precedent synthetic system was developed with two different inducible states, an “on” and “off” state. The system was created using two promoter and repressor pairs. Both repressors were inhibited by a different inducer (aTc and IPTG). The promoters were placed upstream of the repressor gene of the opposite pair. This created a bistable system where only one promoter would be expressed at any one time, since the expression of a promoter repressed expression of the other promoter. To distinguish a cell between its on state and off state, a green fluorescence protein transcription gene was placed downstream of the on state promoter so the cell would exhibit green fluorescence in its on state. Text legend for the figure APPLICATION OF MY PROPOSED NEW DEVICE Design of a New Device Building the New Device SYNTHETIC DNA LAYOUT RESOURCES TYPE IIS ASSEMBLY Testing the New Device LAC OPERON MODEL SIMULATION I used a model of the natural Lac operon to learn how changing the parameter values changes the behavior of the system. RELATIONSHIP BETWEEN THE LAC MODEL AND MY NEW DESIGN Similarities: Differences: TESTING THE NEW DEVICE