User:Melvin Colorado Escobar/Notebook/chem 471/2015/09/23: Difference between revisions
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==Objective== | ==Objective== | ||
* To learn about Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC) and X-ray Powder Diffraction (XRD). Additionally, for this lab we'll be working on those devices to get spectra for the lysozyme AuNP fibers (using the 5 mL) fiber samples that we have prepared previously. | |||
==Description== | ==Description== | ||
* Before preparing the appropriate samples for each device, all 10 tubes of fibers were transferred into 5 falcon tubes. The fibers were centrifuged for 5 mins at 4000 R. These centrifuged fibers were used to prepare samples for each device. | |||
- 1st: Sample prepration for XRD: | |||
* Drops of fibers were added into the appropriate amount of PlayDo and mixed together. The fiber-PlayDo sample was placed into the special lid for XRD. | |||
- 2nd: Sample preparation for DSC: | |||
* The masses of 4 lids and pans were recorded. | |||
* 2 lids and pans were used for the fiber and the other two were used for lysozyme. | |||
* For fiber: | |||
Sample 1 | |||
# lid and pan mass = 51.37 mg | |||
#sample mass = 3.49 mg | |||
#sample+lid+pan mass = 53.73 mg | |||
Sample 2 | |||
#lid and pan mass = 50.80 mg | |||
#sample mass = 4.43 mg | |||
#sample+lid+pan mass = 53.74 mg | |||
* For lysozyme: | |||
Sample 3 | |||
#lid and pan mass = 51.237 mg | |||
#sample mass = 3.04 mg | |||
#sample+lid+pan mass = 54.38 mg | |||
Sample 4 | |||
#lid and pan mass = 50.71 mg | |||
#sample mass = 3.63 mg | |||
#sample+lid+pan mass = 54.44 mg | |||
* The four samples were pressed three times using a pressor. | |||
* Sample 1 was placed in well #27 in the DSC. The samples 2, 3 and 4 were placed in wells 28, 29 and 30. | |||
* The cycling procedure: | |||
#Equilibrate at 20°C | |||
#Ramp up to 5°C/min and to 100°C. | |||
#Isothermal - 3 mins | |||
#Ramp down to 5°C/min and to 20°C | |||
#Repeat (blue arrow) | |||
#Lastly, external event (OFF) | |||
==Data== | ==Data== | ||
* Add data and results here... | * Add data and results here... |
Revision as of 11:20, 26 November 2015
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Objective
Description
- 1st: Sample prepration for XRD:
- 2nd: Sample preparation for DSC:
Sample 1
Sample 2
Sample 3
Sample 4
Data
NotesThis area is for any observations or conclusions that you would like to note.
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