User:Slokwong

Name: Sophie Wong
Major: Course 20
Email: slokwong AT mit DOT edu

User:Slokwong/MODULE 1 : Genome Engineering

Ada subunits	size,chromosome,null p-type	notes
Ada1 (aka HFI1, SUP110, SRM12, GAN1)	1.467 kb/489 aa, Chr. XVI, viable
Ada2 (aka SWI8)	1.305 kb/434aa, Chr. IV, viable	==gene product==: Ada histone acetyltransferase complex component, transcriptional coactivator ==molecular function==: transcription coactivator activity (TAS) ==systematic deletion==: viable decreased drug resistance (rapamycin) decreased drug resistance (wortmannin)
Ada3(aka NGG1, SWI7)	2.109 kb/702aa, Chr. IV, viable
Gcn5 (aka ADA4, SWI9)	1.32 kb/439aa, Chr. VII, viable	==gene product== Ada histone acetyltransferase complex component molecular function histone acetyltransferase activity (IDA) transcription coactivator activity (TAS) systematic deletion reduced fitness in rich medium (YPD)
Ada5 (aka SPT20)	1.815 kb/604aa, Chr. XV, viable	same as SPT20

Spt subunits	size, chromosome, null p-type	notes
Spt3	1.014 kb/337aa, Chr. IV, viable
Spt7(aka GIT2)	3.999 kb/1332aa, Chr. II, viable
Spt8	1.809 kb/602aa, Chr. XII, viable	==gene product== transcription factor molecular function TATA-binding protein binding (IDA, IMP) transcription cofactor activity (IMP, IPI) Systematic deletion viable Exhibits sensitivity at 5 generations when grown in synthetic complete - trp medium. normal chitin levels increased drug resistance (rapamycin) decreased drug resistance (wortmannin)
Spt20 (aka Ada5)	1.815 kb/604aa, Chr. XV, viable	==gene product== histone acetyltransferase SAGA complex member transcription factor ==molecular function== transcription cofactor activity (IGI, IMP, IPI) systematic deletions decreased drug resistance (rapamycin) decreased drug resistance (wortmannin)

TAF subunits	size, chromosome, null p-type	notes
TAF5 (aka TAF90)	2.397 kb/798aa, Chr. II, inviable
TAF6 (aka TAF60)	1.551 kb/516aa, Chr. VII, inviable
TAF9 (aka TAF17)	0.474 kb/157aa, Chr. XIII, inviable
TAF10 (aka TAF23, TAF25)	0.621 kb/206aa, Chr. IV, inviable	==gene product== TFIID subunit TafII25 ==molecular function== * general RNA polymerase II transcription factor activity (TAS) ==systematic deletion== * inviable
TAF12(aka TAF61, TAF68)	1.620 kb/539aa, Chr. IV, inviable

Tra1 subunit	size, chromosome, null p-type	notes
Tra1	11.235 kb/3744aa, Chr. VIII, inviable

other subunits	size, chromosome, null p-type	notes
Sgf73	1.974 kb/657aa, Chr. VII , viable
Sgf29	0.779 kb/259aa, Chr. III, viable
Sgf11	0.3 kb/99aa, Chr.XVI, viable
Ubp8	1.416 kb/471aa, Chr. XIII, viable
Sus1	gene with intron, Chr. II, viable