IGEM:PennState

The bacterial relay race takes advantage of an ability to control cellular motility using inducible promoters such as those involved in nutrient catabolism or quorum sensing. “Receiver” bacteria move in response to small-molecule signals either added to the system or originating from motile, “sender” strains. The most significant challenges relating to this project stem from difficulties of tightly controlling the target motility gene motB. Low levels of motB expression result in system failure (constitutive motility), and resolving this issue is essential to developing reliable modular systems that are the hallmark of synthetic biology. >People Undergraduate Team Jon Badalamenti Lucien Weiss Galen Lynch Chris Buckno Joel Q. Grim Ralph Sandoval Barbara Andersen Jen Dunning Natalie Shewski John Bigus Aleksandr Lewicki Elizabeth Morin Yan Shipelskiy Affiliated Grad Students Steve Walker Deepti Tanjore Faculty Tom Richard Pat Cirino Will Hancock Darryl Farber Ming Tien Paul Weiss Graduated Team Members Lane J. Weaver Tony Tascone Steve Neuman Photos Lab Photos Team Member Photos Motility Assay Results Graphics Lab Notebooks Lucien Weiss >Project Publications Synthetic Sports: A Bacterial Relay Race PSU iGEM Posters News Website Tasks Protocols Cloning Preparing chemically competent cells Growing Parts Restriction Ligation Transformation Plate-reading Motility-assays Microchannel Strain Construction Inventory DNA Gel Growth Media LB Plates Eiken Agar Plates Progress Submitted Registry Parts Strain-Construction Progress Microchannels results Motility results Background Development of the Ecoli Relay Race Wikipedia: Relay Race Links iGEM on OpenWetWare Parts registry iGEM wiki Penn State iGEM 2005 Penn State iGEM 2006