IGEM:Peking/2007: Difference between revisions
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Revision as of 17:11, 2 July 2007
 Hop counting is a cell-cell communication system in which the signals transfered among the cells record the number of times of transfer. As a prerequisite for hop counting, cell-cell communication should be made on a one to one basis to ensure differential control over neighboring cells. The two possible mechanism of signal transfer are bacterial conjugation and phage. Although a hop count system can be implemented with some modifications to either of the natural systems, we tend to focus on conjugation while doing some basic work on phage. With the conjugation, independent tandem oriT (<bbpart>BBa_J01002</bbpart> and more) pairs can be inserted into the same plasmids, losing one tandem region at each single conjugation event. Sequential conjugation and deletion of the different oriT pairs can be achieved by fine control of the expression of the cis-acting elements of the independent conjugation systems. Besides intellectual interests, these systems are the core parts of controllable and encoded local cell-cell communication, which can be used in potential pattern formation and material weaving applications. Push-on-push-off Switch |
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