IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-7: Difference between revisions
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*select Positive OriT_J23066_OriT_pSB1A2 Colonies from Plate, Culture in liquid LB,waiting for mini-prep. | *select Positive OriT_J23066_OriT_pSB1A2 Colonies from Plate, Culture in liquid LB,waiting for mini-prep. | ||
==transformation OriT_J23066_OriT_pSB1A2 ( | ==transformation OriT_J23066_OriT_pSB1A2 (normal T4 ligase)== | ||
*transformation OriT_J23066_OriT_pSB1A2 ligate by ''nomal T4 ligase'' . | *transformation OriT_J23066_OriT_pSB1A2 ligate by ''nomal T4 ligase'' . | ||
*Culture at Amp+ plate for 12 hours. | *Culture at Amp+ plate for 12 hours. | ||
Revision as of 13:36, 12 August 2007
Tandem OriT by Qu Mingzhi
Amplification Culture of OriT_J23066_OriT_pSB1A2(fast T4 ligase)
- select Positive OriT_J23066_OriT_pSB1A2 Colonies from Plate, Culture in liquid LB,waiting for mini-prep.
transformation OriT_J23066_OriT_pSB1A2 (normal T4 ligase)
- transformation OriT_J23066_OriT_pSB1A2 ligate by nomal T4 ligase .
- Culture at Amp+ plate for 12 hours.
- NEXT DAY: 200+ cloney received.
- F-OriT_J23066_OriT_pSB1A2 self-ligation nagetive-control received no clones.
