IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-22: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Qu Mingzhi (talk | contribs) New page: ==Amplification Culture of PlacI-I741051-E0240_pSB1A2, R0010-E0240_pSB1A2 (III)== *Amplification Culture of PlacI-I741051-E0240_pSB1A2, R0010-E0240_pSB1A2 from plates. ==mini-prep PlacI-I... |
Qu Mingzhi (talk | contribs) |
||
| Line 8: | Line 8: | ||
*Digesting PlacI-I741051-E0240_pSB1A2, R0010-E0240_pSB1A2 with EcoRI/PstI. | *Digesting PlacI-I741051-E0240_pSB1A2, R0010-E0240_pSB1A2 with EcoRI/PstI. | ||
*Digestion system contains： | *Digestion system contains： | ||
<pre> | |||
1 µl 10*H | |||
0.25 µl EcoRI | |||
0.25 µl PstI | |||
5 µl Plasmid | |||
3.5 µl dH20 | |||
-------------------------- | |||
40 µl Total | |||
</pre> | |||
====electrophoresis result==== | |||
from ''left'' to ''right'': | |||
Revision as of 06:51, 23 August 2007
Amplification Culture of PlacI-I741051-E0240_pSB1A2, R0010-E0240_pSB1A2 (III)
- Amplification Culture of PlacI-I741051-E0240_pSB1A2, R0010-E0240_pSB1A2 from plates.
mini-prep PlacI-I741051-E0240_pSB1A2, R0010-E0240_pSB1A2 (III)
- using Transgen mini plasmid puriflication kit.
- 50µL after purflication.
mini-prep double digesting test
- Digesting PlacI-I741051-E0240_pSB1A2, R0010-E0240_pSB1A2 with EcoRI/PstI.
- Digestion system contains：
1 µl 10*H 0.25 µl EcoRI 0.25 µl PstI 5 µl Plasmid 3.5 µl dH20 -------------------------- 40 µl Total
electrophoresis result
from left to right:
