Synthetic Biology:Vectors/Parts: Difference between revisions
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==Replication origins== | ==Replication origins== | ||
* | *<bbpart>BBa_I50000</bbpart>: F plasmid backbone with BioBricks restriction sites removed | ||
* | *<bbpart>BBa_I50010</bbpart>: oriV origin which requires TrfA protein to be functional. | ||
* | *<bbpart>BBa_I50020</bbpart>: high copy origin of replication from pSB1A3 | ||
==Antibiotic resistance cassettes== | ==Antibiotic resistance cassettes== | ||
* | *<bbpart>BBa_P1001</bbpart>: cassette providing tetracycline resistance. | ||
* | *<bbpart>BBa_P1000</bbpart>: cassette providing chloramphenicol resistance. | ||
* | *<bbpart>BBa_P1002</bbpart>: cassette providing ampicillin resistance. | ||
* | *<bbpart>BBa_P1003</bbpart>: cassette providing kanamycin resistance. | ||
==Terminators== | ==Terminators== | ||
* | *<bbpart>BBa_B0055</bbpart>: upstream flanking terminator | ||
* | *<bbpart>BBa_B0054</bbpart>: downstream flanking terminator | ||
===Notes=== | ===Notes=== | ||
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==Others== | ==Others== | ||
* | *<bbpart> BBa_I1000</bbpart> (old) or <bbpart>BBa_P1010</bbpart> (preferred): ccd operon in BioBricks format | ||
*unique identifier for the vector | *unique identifier for the vector | ||
Revision as of 17:54, 23 January 2006
This is a list of BioBricks parts for use in construction of modular vectors.
Some have just been designed while others have been constructed and tested. See the associated registry page for details.
Replication origins
- <bbpart>BBa_I50000</bbpart>: F plasmid backbone with BioBricks restriction sites removed
- <bbpart>BBa_I50010</bbpart>: oriV origin which requires TrfA protein to be functional.
- <bbpart>BBa_I50020</bbpart>: high copy origin of replication from pSB1A3
Antibiotic resistance cassettes
- <bbpart>BBa_P1001</bbpart>: cassette providing tetracycline resistance.
- <bbpart>BBa_P1000</bbpart>: cassette providing chloramphenicol resistance.
- <bbpart>BBa_P1002</bbpart>: cassette providing ampicillin resistance.
- <bbpart>BBa_P1003</bbpart>: cassette providing kanamycin resistance.
Terminators
- <bbpart>BBa_B0055</bbpart>: upstream flanking terminator
- <bbpart>BBa_B0054</bbpart>: downstream flanking terminator
Notes
It wasn't clear from the website if these were bi-directional? Not sure that this is very important--BC
- I don't know if the terminators are bidirectional. These terminators are used as flanking terminators in other vectors and are claimed to make the cloning of difficult pieces of DNA (like strong promoters) easier. This is why I was planning on orienting the antibiotic resistance cassette in the opposite direction so that read through from upstream of the multiple cloning site is less of an issue. -- RS
Others
- <bbpart> BBa_I1000</bbpart> (old) or <bbpart>BBa_P1010</bbpart> (preferred): ccd operon in BioBricks format
- unique identifier for the vector
