Skatebro:Primer Design: Difference between revisions

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[[Skatebro:Notebook08]]


==Tips==
*In general, want highest GC content possible
*Insert a double stop codon (taa taa)
*Binding length of 18-22 bps is ideal plus tails
*Melt temps. of forward and reverse primers should be within ~2c and ~50c is desirable

Latest revision as of 10:08, 9 February 2008

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