IGEM:Cambridge/2008/Notebook/Voltage/OD600 Calibration: Difference between revisions
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=Method= | |||
*A culture of cells was diluted down and the OD600 reading was taken for each. | |||
*Some of the dilutions were plated out at 10<sup>-5</sup> and 10<sup>-6</sup> fold dilutions, and colonies were counted to determine the total number of cells in the original dilutions. | |||
=Results= | |||
[[Image:0D600_curve.JPG | 800px]] | [[Image:0D600_curve.JPG | 800px]] | ||
Revision as of 15:36, 31 July 2008
Method
- A culture of cells was diluted down and the OD600 reading was taken for each.
- Some of the dilutions were plated out at 10-5 and 10-6 fold dilutions, and colonies were counted to determine the total number of cells in the original dilutions.
Results
