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{{Imperial/StartPage2}}__NOTOC__
{{Imperial/StartPage2}}__NOTOC__
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&nbsp; <font size=6px color=#E5EBFF><b>Cloning Strategy</b></font>
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{{Imperial/Box1||The Imperial iGEM 2008 team faces the task of working with a chassis that has been rarely used - and never characterised - in the competition to date. While the ''B. subtilis'' chassis offers us many advantages, working from the ground up presents many challenges.


Our cloning strategy is complex. In order to build the required constructs for our final product, we need to build, test and characterise intermediary parts and devices that will lead to the final system. The diagram below shows the critical pathway for our cloning strategy with a large number of closely-linked steps.
{{Imperial/Box2|Cloning Strategy|
The Imperial iGEM 2008 team faces the task of working with a chassis that has been rarely used - and never characterised - in the competition to date. While the ''B. subtilis'' chassis offers us many advantages, working from the ground up also presents many challenges.}}


{{Imperial/Box1||
The cloning strategy for our Biofabricator is complex. In order to build the required constructs for our final product, we need to build, test and characterise intermediary parts and devices that will lead to the final system. The diagram below shows the critical pathway for our cloning strategy with a large number of closely-linked steps.
<br><br>
[[Image:Imperial_2008_Critical_Pathway.png|center|700px]]
[[Image:Imperial_2008_Critical_Pathway.png|center|700px]]
<br><br>}}


A summary of the aims of each phase and constructs to be produced within each phase, is given below:}}
{{Imperial/Box1|Constructs|
A summary of the aims of each phase and constructs to be produced within each phase, is given below:


====== Phase 1: Testing of Constitutive Promoters ======
====== Phase 1: Testing of Constitutive Promoters ======
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Combination of light sensing and light-induced expression of epsE and biomaterial. Each gene has its own promoter/RBS pair because in ''B. subtilis'', it has been shown that levels of expression decrease as one moves along an operon.
Combination of light sensing and light-induced expression of epsE and biomaterial. Each gene has its own promoter/RBS pair because in ''B. subtilis'', it has been shown that levels of expression decrease as one moves along an operon.
<p><html><img width="100%" src="http://i59.photobucket.com/albums/g305/Timpski/S1L.png"></html></p>
<p><html><img width="100%" src="http://i59.photobucket.com/albums/g305/Timpski/S1L.png"></html></p>
 
}}
{{Imperial/EndPage|Wet_Lab|Protocols}}
{{Imperial/EndPage|Wet_Lab|Protocols}}

Latest revision as of 10:25, 6 October 2008

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Cloning Strategy

The Imperial iGEM 2008 team faces the task of working with a chassis that has been rarely used - and never characterised - in the competition to date. While the B. subtilis chassis offers us many advantages, working from the ground up also presents many challenges.


The cloning strategy for our Biofabricator is complex. In order to build the required constructs for our final product, we need to build, test and characterise intermediary parts and devices that will lead to the final system. The diagram below shows the critical pathway for our cloning strategy with a large number of closely-linked steps.




Constructs


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