IGEM:MIT/2005/Receiver Head Unit: scFv: Difference between revisions

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>Jenny
>Jenny
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==Need Help==
==Need Help==
How to design primers?
Open Issues.

Revision as of 18:14, 12 July 2005

POC

Jenny

Purpose

To provide a landing site for ligand.
To cause dimerization/conformational change in the Receiver. To transmit signal to Receiver.

Current Status

pCT-4-4-20, pCT302 S101A, pCT-4M5.3 anti-fluorescein plasmids miniprepped
Trying to design primers to PCR in biobrick format

Open Issues

  • TAG (myc) to know that the fluorescein has bound.
  • How to design primers so that the sequence is in frame?

Need Help

Open Issues.