PrbbBB:Protocols: Difference between revisions
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* [[Prbbbb:small_scale_expression_v1|Small-scale expression in BL21]] | * [[Prbbbb:small_scale_expression_v1|Small-scale expression in BL21]] | ||
* [[Prbbbb:inclusion_body_solubilization_v1|Solubilization of E. coli inclusion bodies]] | |||
* [[Prbbbb:Coomassi_Ethanol_stain|Coomassi protein stain (Ethanol-based)]] | * [[Prbbbb:Coomassi_Ethanol_stain|Coomassi protein stain (Ethanol-based)]] | ||
[[Category:Protocol]] | [[Category:Protocol]] | ||
Revision as of 08:47, 26 August 2009
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Biobrick assembly & related
We have streamlined the standard Biobrick assembly protocols. Our "Barcelona set" of protocols is optimized for performing many assemblies in parallel.
- prepare vector backbone for assembly (RFC 25 Fusion format)
- Assembly protocol for BioBricks on 96 well plate (RFC 25 Fusion format)
- Colony PCR screening of many assemblies in parallel (on 96 well plate)
- Ligation-free Biobrick Construction by PCR/recombination (RFC 25 Fusion format)
- DNA measurement on plates
