SBB10Ntbk-JoseGutierrez: Difference between revisions
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[[Template:SBB10Project- | [[Template:SBB10Project-37738 | My Project]]<br> | ||
=Construction Files= | =Construction Files= | ||
==SBB30: 5'UTR_rep009== | ==SBB30: 5'UTR_rep009== | ||
Revision as of 00:50, 16 April 2010
Construction Files
SBB30: 5'UTR_rep009
Biobricking of O99 rep Gene
PCR JG001-F and JG002-R on pEC52 (1235 bp, EcoRI/BamHI)
Sub into pBca9523-Bca1144 (EcoRI/BamHI, 910+2472, L)
Product is pBca9523-sbb30 {5'UTR_repO99!}
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JG001-F Cloning of O99 rep Gene
ccataGAATTCatgAGATCTaaaaacgattctgacgcattttttatg
JG002-R Cloning of O99 rep Gene
CTGATGGATCCCTACTCTACAAGACCTCGTTTTttc
SBB38: PBad Promoter
Eco/Bam transfer pBjh1601CK-ig114
Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-ig114 {AraC-Pbad}
SBB37: PCon Promoter
Eco/Bam transfer pBca1100-Bca1152
Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-Bca1152 {Pcon-J23100}
