Stanford/BIOE44:Module 1:Day6: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 17: | Line 17: | ||
==Some interesting results for those doing a colony PCR== | ==Some interesting results for those doing a colony PCR== | ||
[[Image:AnotatedAdministrator 2010-04-20 00hr 39min.jpg]] | [[Image:AnotatedAdministrator 2010-04-20 00hr 39min.jpg]] | ||
1% and 0.8% agarose (90V) | |||
''' | |||
What can you gather from this gel? | |||
''' | |||
Summary of Procedures | |||
1) Colony PCR, arsR biobrick primers, MG1655 | |||
2) PCR Cleanup Qiagen | |||
3) Digestion with EcoRi and SpeI | |||
Method A: PCR Cleanup Kit only | |||
Method B: PCR Cleanup Kit + Gel Extraction |
Revision as of 11:01, 20 April 2010
M1: Day 6 - Arsenic!
How to find and get parts
- Search the parts registry.
- When you find a part you want, click on the "get this part" link located on the right.
- If the part is in the 2009 or 2007 distribution, we should have it (but there is a small chance we won't if it has already been used).
- Send an email to Isis with the following information. All of this info is listed on the 'Get Part' page!:
- Part number
- Distribution (ex. Spring 2007)
- Plate number
- Well number
- Antibiotic resistance
- Isis will send you a confirmation email containing which parts are available to you.
- Isis will transform the parts into E. coli. You will receive a plate with colonies on it. This means it will take at least a day to get your parts.
Some interesting results for those doing a colony PCR
1% and 0.8% agarose (90V) What can you gather from this gel?
Summary of Procedures
1) Colony PCR, arsR biobrick primers, MG1655 2) PCR Cleanup Qiagen 3) Digestion with EcoRi and SpeI
Method A: PCR Cleanup Kit only Method B: PCR Cleanup Kit + Gel Extraction