Yu:TBS

Solution recipe for 1L 5x Tris-Buffered Saline. Dilute to 1x for use.

1. Measure 700mL millipore water into a 2L plastic beaker.	5x
2. Weigh and add 43.83g NaCl, or add 150mL 5M NaCl	750mM NaCl
3. Add 50mL 1M TRIS-HCl pH 7.5	50mM TRIS pH 7.5
4. Stir until dissolved
5. Bring volume to 1L with millipore water in a graduated cylinder
6. Dilute from 5x to 1x (1:5) for use

Solution recipe for 1L 5x Tris-Buffered Saline / Tween-Triton. Dilute to 1x for use.

1. Measure 700mL millipore water into a 2L plastic beaker.	5x
2. Weigh and add 146.1g NaCl, or add 500mL 5M NaCl	2.5M NaCl
3. Add 100mL 1M TRIS-HCl pH 7.5	100mM TRIS pH 7.5
4. Add 2.5mL Tween-20	0.25% Tween-20
5. Add 10mL Triton-X100	1% Triton-X100
6. Stir until dissolved
7. Bring volume to 1L with millipore water in a graduated cylinder
8. Dilute from 5x to 1x (1:5) for use