PrbbBB:Protocols: Difference between revisions
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==Protein measurements== | ==Protein measurements== | ||
* [[Prbbbb:in_vitro_FRET_FRB_FKBP_v1|''in-vitro'' FRET measurements]] | |||
[[Category:Protocol]] | [[Category:Protocol]] | ||
Revision as of 15:29, 25 May 2010
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Biobrick assembly & related
We have streamlined the standard Biobrick assembly protocols. Our "Barcelona set" of protocols is optimized for performing many assemblies in parallel.
- prepare vector backbone for assembly (RFC 25 Fusion format)
- Assembly protocol for BioBricks on 96 well plate (RFC 25 Fusion format)
- Colony PCR screening of many assemblies in parallel (on 96 well plate)
- Ligation-free Biobrick Construction by PCR/recombination (RFC 25 Fusion format)
- DNA measurement on plates
Protein Expression
- Small-scale expression in BL21
- Large-scale expression in BL21
- Solubilization of E. coli inclusion bodies
- Coomassi protein stain (Ethanol-based)
