IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/07/24: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;">Memo cell</span> | |||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |||
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Population counter</span> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Population counter</span> | ||
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*TetA(C) (J31007) | *TetA(C) (J31007) | ||
br><u>Restriction digest</u> of the same minipreps as yestarday (concentration measured by nanodrop 07/23), around 3µg of DNA per reaction. | <br><u>Restriction digest</u> of the same minipreps as yestarday (concentration measured by nanodrop 07/23), around 3µg of DNA per reaction. | ||
*term/attC/mRFP : 42µl DNA + 5µl Buffer 3 10x + 0.5µl BSA 100x + 2µl XbaI + 2µl PstI | *term/attC/mRFP : 42µl DNA + 5µl Buffer 3 10x + 0.5µl BSA 100x + 2µl XbaI + 2µl PstI | ||
*attC/term/attC/mRFP : 42µl DNA + 5µl Buffer 3 10x + 0.5µl BSA 100x + 2µl XbaI + 2µl PstI | *attC/term/attC/mRFP : 42µl DNA + 5µl Buffer 3 10x + 0.5µl BSA 100x + 2µl XbaI + 2µl PstI | ||
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*attC/term/attC/mRFP : 25µl + 5µl lb | *attC/term/attC/mRFP : 25µl + 5µl lb | ||
*- | *- | ||
* | *term/attC/mRFP : 25µl + 5µl lb | ||
* | *term/attC/mRFP : 25µl + 5µl lb | ||
[[Image:gel2407_1.jpg]] | [[Image:gel2407_1.jpg]] | ||
The absence of the band corresponding to the insert for attC/term/attC/mRFP raises some questions about the real nature of this plasmid... | [[Image:gel2407_2.jpg]] | ||
<br>The absence of the band corresponding to the insert for attC/term/attC/mRFP raises some questions about the real nature of this plasmid... | |||
<br><u>Gel purification</u> with the Quiagen kit. | <br><u>Gel purification</u> with the Quiagen kit. | ||
<br>All bands are eluted with 30μL of water. | <br>All bands are eluted with 30μL of water. | ||
<br><u>Overnight cultures</u> of the transformations from 07/23 (all | <br><u>Overnight cultures</u> of the transformations from 07/23 (colonies on all plates): | ||
<br> 2x20mL for each transformation | |||
*TOP10 pSB1A2 weak RBS -TetA(C) - Amp | *TOP10 pSB1A2 weak RBS -TetA(C) - Amp | ||
*TOP10 pSB1A2 med.RBS -TetA(C) - Amp | *TOP10 pSB1A2 med.RBS -TetA(C) - Amp |
Revision as of 06:18, 5 August 2010
Memo cell | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||
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Population counter | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||
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