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| =Embryo details= | | =Embryo details= |
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| <br>Species: Gallus gallus domesticus | | <br>'''Species''': |
| <br>Embryo Name:RC-009 | | <br>'''Embryo Name''': |
| <br>Embryo stage: 24 {{Note|Staging not confirmed by Supervisor}} | | <br>'''Embryo stage''': |
| <br>Staging description: | | <br>'''Staging description''': |
| *Elbow and knee joints are distinct. This may indicate that the embryo is greater than stage 24.
| | <br>'''Fixation''': |
| *No toe demarcation visible. This may indicate that the embryo is less than stage 26.
| | <br>'''Cryoprotection''': |
| *Toe groove on developing leg not visible. This indicates that the embryo has not reached stage 25.
| | <br>'''Material label and storage''': |
| Therefore the embryo is stage 24.
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| <br>Cryoprotection: Not cryoprotected. Sectioned from PFA. | |
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| =Experiment details= | | =Experiment details= |
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| <br>Objective: Analyze Nissl-stained serial sections of the embryo. | | <br>'''Objective''': |
| <br>Procedure: | | <br>'''Procedure''': |
| *Staged the embryo according to Hamburger and Hamilton staging (1951). {{Note|This is an approximation as it was not checked}}
| | <br>'''Comments''': |
| *Under a dissection microscope the head was cleaved from the tail using the dissection scissors at the level of the wing bud. {{Note|This can not be confirmed as there was no photograph taken of the embryo prior to cutting}}
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| *The embryo was embedded in OCT and frozen at -19°C (in the cryostat chamber), loaded onto a chuck and incubated for an hour.
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| *Serial sections were cut and mounted onto the gelatin-subbed slides whilst time spent cutting the section and time spent in chamber before mounting were recorded into my lab-book.
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| *The sections were dried and then Nissl-stained for visualization under a microscope.
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| <br>Comments:I did not allow myself enough time to complete the sectioning of the embryo. I stopped halfway through the hindbrain. The other half of the hindbrain and head were discarded. | |
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| =Results= | | =Results= |
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| Half of the hindbrain and head are missing from the slides as they were discarded.
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| =Images= | | =Images= |
| | =Summary= |
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| No images have been taken.
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| =Summary=
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| The objective was not achieved as I did not allow enough time to complete the cutting of the embryo.
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| __NOTOC__
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| Cranial Nerve Development
|
Experiment
|
Embryo details
Species:
Embryo Name:
Embryo stage:
Staging description:
Fixation:
Cryoprotection:
Material label and storage:
Experiment details
Objective:
Procedure:
Comments:
Results
Images
Summary
