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| == '''BISC110/112 Assignments/ Weekly Lab Planner''' ==
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| {| border=1px
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| |'''Series'''
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| |'''Lab #'''
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| |'''LAB DATES'''
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| |'''LAB DESCRIPTION'''
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| |'''Assignment/Notes'''
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| |--
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| | colspan="7" |
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| |--
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| | 1
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| | 1
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| | Jan. 24 to<br> Jan. 28
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| | Practice metric conversions; <BR>Practice pipetting; <BR>Making Solutions; Microscopy; <BR>Examination of ''Tetrahymena pyriformis''<br>
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| | '''Homework''': Familiarize yourself with the wiki, particularly the information in the [[BISC110/S11:Resources]] section of the Lab wiki; <BR>Lab 1 Practice Problems downloadable here [[Media:Lab_1_Practice_Problems110.doc]];<br>
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| Read about the process of endocytosis in your textbook; familiarize yourself with phagocytosis in ''Tetrahymena'' from the following reference articles:<BR> '''*Gronlien HK, Berg T, Lovlie AM.''' In the polymorphic ciliate ''Tetrahymena vorax'', the non-selective phagocytosis seen in microstomes changes to a highly selective process in macrostomes. (2002), Journal of Experimental Biology 205, 2089-2097; <BR>
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| '''*McLaughlin NB and Buhse HE, Jr.,''' Localization by indirect immunofluorescence of tetrin, actin, and centrin to the oral apparatus and buccal cavity of the macrostomal form of ''Tetrahymena vorax''., (2004), J Eukaryot. Microbiol., 51(2), 253-257.
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| |--
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| | 1
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| | 2
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| | Jan. 31 to<br> Feb. 4
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| | ''Tetrahymena'' Behavior: Phagocytosis; Observations; Data Analysis; Accuracy vs. Precision; Statistical Parameters; Data Presentation; Effective Figure Design and Legends using the Gronlien article as a model.
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| | '''Homework''': Study dilution making for Lab 3; <BR>Generate two figures with legends from your ''Tetrahymena'' experiment: one or more microphotographs of Tetrahymena and a graph or table of data.
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| |--
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| | 1
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| | 3
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| | Feb. 7 to<br> Feb. 11
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| | Making Dilutions; Determination of toxicity of Heavy Metals on ''Tetrahymena'';<BR> Design your own experiment exploring inhibitors and ''Tetrahymena'' phagocytosis
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| | '''Homework''': There are animated tutorials on dilution<BR> available at the following link: [http://www.wellesley.edu/Biology/Concepts/Html/dilutions.html Dilution Help Tutorials]; <br> Finalize your experimental design for Lab 4; have it approved by your lab instructor;<br> Write a results section of a scientific report on your findings in the ''Tetrahymena'' toxicity experiment including a narrative portion and integrated figures with legends;<br>
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| Work the dilution problems found at [[Media: Lab_3_110_Dilution_Problems.doc]] and hand them in in Lab 4.
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| |--
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| | 1
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| | 4
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| | Feb. 14 to <br> Feb. 18
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| | Student Designed Experiments:<BR>Potential Inhibitors of Vacuole Formation;
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| | '''Homework''': 5 minute oral presentation in PowerPoint of your data, including a figure and figure legend, summarizing your Lab 4 experimental results. <br>Read assigned journal article for science writing discussion <BR>'''* Gronlien, H.K., Berg, T., Lovlie, A.M.''' (2002). In the polymorphic ciliate ''Tetrahymena vorax'', the non-selective phagocytosis seen in microstomes changes to a highly selective process in macrostomes, J. Exp. Biol. 205, 2089-2097
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| |--
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| | 1
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| | 5
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| | Feb. 22 to <br> Feb. 28
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| | Scientific Writing Workshop with Journal Article Discussion;<BR> Science Writing Workshop;
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| | '''Homework''': '''''Tetrahymena'' Partial Scientific Research Report: DUE AT THE BEGINNING OF LAB 6'''<BR> Title Page, Results, Discussion and References sections. <BR>Guidelines at:[[BISC110/S11:Resources | Resources]]; <BR>Science Writing peer-tutor appointment scheduling at: [http://www.wellesley.edu/Writing/Program/tutors.html Wellesley College Writing Program Resources].<br>
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| Have Lab notebooks ready to be turned in for inspection after Lab 6.
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| <br> Read Lab 6 (first lab in Series 2, Genetics)
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| <br>Prepare a flow chart in your lab notebook of all Lab 6 experimental procedures.
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| |--
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| | 2
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| | 6
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| | March 1 to<br> March 7
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| | Genetics: Taster SNP 1-Test PTC Taster Phenotype;<BR> Isolate and digest DNA; PCR;
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| | '''Homework''': Read the following background information on [http://serc.carleton.edu/files/genomics/units/snp_restriction_enzymes.pdf restriction enzymes] and on [http://serc.carleton.edu/files/genomics/units/snp_gel_background.pdf gel electrophoresis] <br>
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| Read the following background information on ethical issues in genetic testing:<br>
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| '''[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2710700/?tool=pubmed Direct-to-Consumer Genetic Testing,''' Two Sides of the Coin] by Farkas and Holland, J Mol Diagn. 2009 Jul;11(4):263-5. Epub 2009 Jun 18. <BR>
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| [http://0-www.sciencemag.org.luna.wellesley.edu/cgi/reprint/320/5872/53.pdf PUBLIC HEALTH: A Case Study of Personalized Medicine] by S. H. Katsanis, G. Javitt, and K. Hudson, published in ''Science'', 4 April 2008 320: 53-54 [DOI: 10.1126/science.1156604](in Policy Forum)<BR>
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| [http://www.dnapolicy.org/news.enews.article.nocategory.php?action=detail&newsletter_id=35&article_id=156 AMA DTC testing policy recommendations] <br>
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| Draw the expected migration locations of the Fnu4H1 digested PCR product obtained from a homozygous taster (left lane), a homozygous nontaster (center lane)<BR> and a heterozygote (right lane) on a diagram of an agarose gel; <br>
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| Answer the questions found at:<BR>[[Media:Taster_DTC_questions110.doc]] to use as a template for your answers.'''
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| |--
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| | colspan="7" |
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| |--
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| | 2
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| | 7
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| | March 8 to <br> March 14
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| | Genetics: Taster SNP 2: Prepare and perform agarose gel electrophoresis;<BR> Determine PTC Taster Genotype; Scientific Writing Workshop
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| | '''Homework: Write a scientific research report (all sections except M&M)on your PTC Taster SNP study.<BR> Due on April 1st at 5pm.'''<BR> For help, see the [[BISC110/S11:Resources | Resources]] section of this wiki.
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| |--
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| | 3
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| | 8
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| | March 28 to<br> April 1
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| | Photosynthesis 1: Photosynthetic pigments
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| | '''Homework''': Prepare a full results section for the photosynthetic pigment identification experiment;<br>
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| In your lab notebook, prepare a flow chart for the Hill Reaction (Lab 9);<br>
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| Review the mechanisms of electron transport and proton flow in photosynthesis (use your textbook).<br>
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| |--
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| | 3
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| | 9
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| | April 4 to<br> April 8
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| | Photosynthesis 2: Hill Reaction
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| | '''Homework''': Finalize your self-designed experiment testing a variable affecting the Hill Reaction;<br> Write a Materials and Methods style version of the Hill Reaction experiment that you did this week in Lab 9
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| |--
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| | 3
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| | 10
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| | April 11 to <br> April 15
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| | Photosynthesis 3: Investigation of variables affecting the Hill Reaction
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| | '''Homework''': Plot your A580 data (y-axis) versus time in seconds (x-axis) for the different treatments (with the treatments on the same graph).See([[Appendix_E/S11 |Appendix E]]). <BR> Determine regression equations for the linear part of each data plots to get slope (m) values (slopes represent the rates of electron transport). <BR>Calculate the rates (change over time of the linear portion of your graphs) and plot those values against your variable (x-axis);<br>
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| '''A full paper on your experiment, in the form of a scientific research report,<BR> is due at beginning of Lab 11.'''<BR> Discuss your results in terms of how the factor that you chose to investigate affects the electron transport rate in photosynthesis.<BR> Compare your results to other investigations published in the primary literature.<BR> Refer to the "[[BISC110/S11:Guidelines for writing a lab report in the form of a scientific paper | '''Guidelines for science writing''']]" information found in the [[BISC110/S11:Resources | Resources]] section of the wiki.
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| |--
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| | 4
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| | 11
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| | April 20 to<br> April 26
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| | Enzymology
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| | '''Homework: Prepare for a Lab Practical Skills Assessment in lab 12.'''<br>
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| Answer the following questions (5 points) and hand them in to your instructor at the beginning of Lab 12. <BR>Download a Word document form of these questions here:[[Media: Lab_11_Questions_110.doc]]
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| <br> Make sure Lab Notebook is ready to turn in for grading
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| |--
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| | Skills Assessment
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| | 12
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| | May 2 to <br> May 6
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| | '''Lab Practical'''; <br>Turn in Lab Notebook
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| | End of lab
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| |--
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| |}
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| <br>
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| =='''Lab Graded Assignments'''== | | =='''Lab Graded Assignments'''== |
BISC 111/113: Introductory Organismal Biology
BISC111/113 Fall 2011 Lab Calendar
| Monday |
Tuesday |
Wednesday |
Thursday |
Friday
|
August 29
|
August 30
Lab 1 |
August 31
Lab 1 |
September 1
Lab 1 |
September 2
Lab 1 |
|
September 5
Labor Day
No Classes
|
September 6
|
September 7
Monday Schedule
Lab 1 |
September 8
Lab 2
|
September 9
Lab 2
|
|
September 12
Lab 2
|
September 13
Lab 2 |
September 14
Lab 2 |
September 15
Lab 3 |
September 16
Lab 3 |
|
September 19
Lab 3
|
September 20
Lab 3
|
September 21
Lab 3 |
September 22
Lab 4 |
September 23
Lab 4 |
|
September 26
Lab 4
|
September 27
Lab 4 |
September 28
Lab 4 |
September29
Lab 5 |
September 30
Lab 5 |
|
October 3
Lab 5
|
October 4
Lab 5 |
October 5
Lab 6 |
October 6
|
October 7
|
|
October 10
Fall Break
No Classes
|
October 11
Fall Break
No Classes |
October 12
Lab 6 |
October 13
Lab 6 |
October 14
Lab 6 |
|
October 17
Lab 6
|
October 18
Lab 6 |
October 19
Lab 7 |
October 20
Lab 7 |
October 21
Lab 7 |
|
October 24
Lab 7
|
October 25
Lab 7 |
October 26
Lab 8 |
October 27
Lab 8 |
October 28
Lab 8 |
|
October 31
|
November 1
Tanner
No Classes |
November 2
Lab 8 |
November 3
Lab 8 |
November 4
Lab 8 |
|
November 7 4
Lab 8
|
November 8
Lab 8 |
November 9
Lab 9 |
November 10
Lab 9 |
November 11
Lab 9 |
|
November 14
Lab 9
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November 15
Lab 9 |
November 16
Lab 11 |
November 17
Lab 11 |
November 18
Lab 11 |
|
November 21
Lab Practical
Lab 10 for all
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November 22
|
November 23
Thanksgiving
Break |
November 24
Thanksgiving
Break |
November 25
Thanksgiving
Break |
|
November 28
Lab 11
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November 29
Lab 11 |
November 30
|
December 1
Lab 12 |
December 12
Lab 12 |
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December 5
Lab 12
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December 6
Lab 12 |
December 7
Lab 12 |
December 8
|
December 9
|
|
Schedule of Experiments
| Section |
Lab # |
Activities
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| 1
|
1
|
Biodiversity
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| 2
|
2
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Population Growth: Designing the Experiment
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| 3
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3-6
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Plant Biology: Transpiration, Anatomy, Photosynthesis
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| 2
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6
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Population Growth: Generation 2
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| 4
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7-9
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Animal Biology: Vertebrate Anatomy, Circulation and Respiration, Nerve Transmission
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| 4
|
10
|
Lab Practical
|
| 2
|
11-12
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Population Growth: Generation 3 and Analysis
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Lab Graded Assignments
| Lab Assigned |
Lab Due |
Assignment
description |
Point Value
(Total 200)
|
| 1
|
2
|
Practice Problems
|
5
|
| 2
|
3
|
Fig. Design (ungraded)
|
0
|
| 3
|
4
|
Dilution Problems (5);
Fig.& Results (10)
|
15
|
| 4
|
5
|
Oral presentation: Results, original figure & legend;
Journal article discussion
|
10
|
| 5
|
6
|
Lab notebook check (ungraded);
Partial Scientific Paper(25)
|
25
|
| 6
|
7
|
SNP Taster Questions
|
5
|
| 7
|
Due for all students
Fri. 4/1 by 5pm
|
Taster Scientific Paper (all but M&M)
|
35
|
| 8
|
9
|
Photosyn. Pigments Results
|
10
|
| 9
|
10
|
M&M (5pt);
Finalize Self-Design
Experiment Hill Rxn.(ungraded)
|
5
|
| 10
|
11
|
Full Scientific Paper Hill Rxn.
|
35
|
| 11
|
12
|
Lab Notebook Check (10)
Concept Questions Enzymology/Gene Reg. (5)
|
15
|
| 12
|
12
|
Lab Practical:Hands-on skills assessment
|
35
|
| Other
|
-
|
Discretionary Points
Preparation & Participation
|
5
|
