Richard Lab:PCR: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
Back to [[Richard Lab:protocols]] | |||
===Overview=== | ===Overview=== | ||
While PCR is typically used for amplification of DNA it can also be used for many other things. Here is a list of the PCR protocols typically used in the Richard Lab. | While PCR is typically used for amplification of DNA it can also be used for many other things. Here is a list of the PCR protocols typically used in the Richard Lab. | ||
| Line 7: | Line 7: | ||
*[[PCR Overlap Extension]] for larger constructs from small parts. | *[[PCR Overlap Extension]] for larger constructs from small parts. | ||
===Mutagenesis=== | ===Mutagenesis=== | ||
*[[Richard Lab:Site Directed Mutagenesis]] | *[[Site-directed mutagenesis|Richard Lab:Site Directed Mutagenesis]] | ||
*Random Mutagenesis | |||
*Saturation Mutagenesis | |||
===Quantification=== | ===Quantification=== | ||
Revision as of 22:25, 14 September 2011
Back to Richard Lab:protocols
Overview
While PCR is typically used for amplification of DNA it can also be used for many other things. Here is a list of the PCR protocols typically used in the Richard Lab.
Amplification
DNA Synthesis
- DNA Synthesis from Oligos for making smaller parts
- PCR Overlap Extension for larger constructs from small parts.
Mutagenesis
- Richard Lab:Site Directed Mutagenesis
- Random Mutagenesis
- Saturation Mutagenesis
