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| ==Materials== | | ==Materials== |
| List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.
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| *supply 1 (i.e. tubes of a certain size? spreaders?)
| | Materials below provided by Dr. J. Cannon. |
| *reagent 1
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| *X μL reagent 2 | | *CHCl3 |
| **component A (reagent 2 is made up of multiple components) | | *MeOH |
| **component B
| | *Lipid solution |
| *equipment 1 | | *Vial |
| *equipment 2 | |
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| ==Procedure== | | ==Procedure== |
| #Step 1
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| #Step 2
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| #*Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
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| #Step 3
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| ##Step 3 has multiple sub-steps within it.
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| ##Enumerate each of those.
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| ==Notes==
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| Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
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| #List troubleshooting tips here.
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| #You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
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| #Anecdotal observations that might be of use to others can also be posted here.
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| Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
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| ==References==
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| '''Relevant papers and books'''
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| <!-- If this protocol has papers or books associated with it, list those references here.-->
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| <!-- See the [[OpenWetWare:Biblio]] page for more information, but this format doesn't work currently.
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| <biblio>
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| #Goldbeter-PNAS-1981 pmid=6947258
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| #Jacob-JMB-1961 pmid=13718526
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| #Ptashne-Genetic-Switch isbn=0879697164
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| </biblio>-->
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| <!-- Try the [[Template:FormatRef|FormatRef template]]-->
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| #{{FormatRef|Goldbeter, A and Koshland, DE|1981| |Proc Natl Acad Sci U S A 78(11)|6840-4| }} PMID 6947258
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| #{{FormatRef|Jacob, F and Monod, J J|1961| |Mol Biol 3(3)|318-56| }} PMID 13718526
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| #{{FormatRef|Ptashne, M|2004|Genetic Switch: Phage Lambda Revisited| | |Cold Spring Harbor Laboratory Press}} ISBN 0879697164
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| ==Contact==
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| *Who has experience with this protocol?
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| or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].
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| <!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. -->
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| <!-- Move the relevant categories above this line to tag your protocol with the label
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| [[Category:Protocol]]
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| [[Category:Needs attention]]
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| [[Category:In vitro]]
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| [[Category:In vivo]]
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| [[Category:In silico]]
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| [[Category:DNA]]
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| [[Category:RNA]]
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| [[Category:Protein]]
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| [[Category:Chemical]]
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| [[Category:Escherichia coli]]
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| [[Category:Yeast]]
| | #Add 6mL CHCl3 and 2mL MeOH for a total of 8mL of 3:1 CHCl3:MeOH. |
| --> | | #Take 2.5mL of CHCl3/MeOH solution and add it to the lipid solution in the vial (will have 2 vials total). |
| | #Use 1mL of lipid/CHCl3/MeOH solution and place it in a 4mL vial (should have 5 vials total). |
| | #Dry the vials using a roto-evaporator. |
| | #Place vial in a desicator overnight to thoroughly dry. |
| | #Store vials in freeze for future use. |
Preparation of Lipid Films
The purpose of this protocol was to prepare lipid films for use in future experiments.
Materials
Materials below provided by Dr. J. Cannon.
- CHCl3
- MeOH
- Lipid solution
- Vial
Procedure
- Add 6mL CHCl3 and 2mL MeOH for a total of 8mL of 3:1 CHCl3:MeOH.
- Take 2.5mL of CHCl3/MeOH solution and add it to the lipid solution in the vial (will have 2 vials total).
- Use 1mL of lipid/CHCl3/MeOH solution and place it in a 4mL vial (should have 5 vials total).
- Dry the vials using a roto-evaporator.
- Place vial in a desicator overnight to thoroughly dry.
- Store vials in freeze for future use.
