Lidstrom:Colony PCR: Difference between revisions
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New page: ==General:== *The thermocycling denaturation & extension temperature should be that recommended by your particular PCR kit. I have seen 68oC & 72oC. *The annealing temperature is up to y... |
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==General:== | ==General:== | ||
*The thermocycling denaturation & extension temperature should be that recommended by your particular PCR kit. I have seen 68oC & 72oC. | *The thermocycling denaturation & extension temperature should be that recommended by your particular PCR kit. I have seen 68oC & 72oC. | ||
Revision as of 23:12, 2 February 2012
Back to Protocols
General:
- The thermocycling denaturation & extension temperature should be that recommended by your particular PCR kit. I have seen 68oC & 72oC.
- The annealing temperature is up to you. For VF2 & VR 54-56oC works well.
- The primer concentration varies in different people's recipes.
