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Revision as of 23:48, 31 August 2012
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<img src="http://openwetware.org/images/3/35/Formattop2.jpg " alt="Mother Cube2" "width="800" height="141"//>
<a href="http://openwetware.org/wiki/Biomod/2012/Tohoku/Team_Sendai ">Team Sendai</a> > <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Top ">Team Sendai A Top</a>> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Project">Project</a>
Project
In order to create the Mother Cube, we need first to explore the method for parallel bonding DNA origami planes.
We think on three bonding methods:"Using lipid as adhesive", "Using sticky ends growing from origami", and "Using lipid and sticky ends".
We think "Using lipid as adhesive" is the best method among the three because there is no need of annealing-time.
<img src="http://openwetware.org/images/5/51/Stage1.jpg" alt="stage1" "width="580" height="23"/>
Studying the interaction between lipid and DNA origami, and creating the following shapes with cadnano.
<a href="http://openwetware.org/wiki/BIOMOD/2012/Team_Sendai_A/CADNANO " target="_blank"> <img src="http://openwetware.org/images/f/f1/Planeshape.jpg" alt="plane shape" border="0" "width="580" height="185"/></a>
If you click this image, you get the DNA sequences.
<img src="http://openwetware.org/images/0/0f/Stage2.jpg" alt="stage2" "width="580" height="27"/>
We have three ideas about what method to follow in order to bond a plane to other DNA origami plane. We are looking for the best method.
<img src="http://openwetware.org/images/2/20/Method.jpg " alt="bond method" "width="580" height="517"/>
<img src="http://openwetware.org/images/b/b9/Stage3.jpg " alt="stage3" "width="580" height="27"/>
Creating the cubes. Loading function and bonding them.
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