BME103:W930 Group5 l2

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Student Role(s): Experimental Protocol Planner Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) LAB 2 WRITE-UP Thermal Cycler Engineering Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski. System Design Key Features Instructions Protocols Materials Supplied in Kit Supplies Amount Micro Test Tubes ? Transfer Pipettes ? DNA Solution 6 samples (600.0μL) Positive Control Solution 1 sample (100.0μL) Negative Control Solution 1 sample (100.0μL) PCR Machine 1 Fluorimeter 1 Included in Fluorimeter Package Supplies Amount Smart phone stand 1 LCD Box 1 Light box 1 Sybr green solution 500.0μL Components of PCR master mix: DNA Solution Component Amount Patient’s Template DNA* 0.2μL 10μM forward primer 1.0μL 10μM reverse primer 1.0μL GoTaq master mix 50.0μL dH2O 47.8μL Total 100.0μL PCR Protocol DNA Measurement Protocol Research and Development Background on Disease Markers Primer Design Illustration