User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/05: Difference between revisions
From OpenWetWare
Dhea Patel (talk | contribs) |
Dhea Patel (talk | contribs) |
||
Line 30: | Line 30: | ||
*Once it was determined that 100μM adenosine created a clear spectra, kinetics was run at 265nm using 2.38mL phosphate buffer, 600μL of 500μM stock adenosine, and 20μL ADA (as outlined in the table above). | *Once it was determined that 100μM adenosine created a clear spectra, kinetics was run at 265nm using 2.38mL phosphate buffer, 600μL of 500μM stock adenosine, and 20μL ADA (as outlined in the table above). | ||
**The velocity was determined by clicking Operations > Activity Table > dAbs. The average of the dAbs values is the average slope (velocity). | **The velocity was determined by clicking Operations > Activity Table > dAbs. The average of the dAbs values is the average slope (velocity). | ||
*Then, an assay was run at 265nm using 2.38mL phosphate buffer, 600μL of 50μM stock adenosine, and 20μL ADA (as outlined in the table above). | |||
**The absorbance was close to 0, indicating that the ideal adenosine concentration ranges between 1μM and 10μM. | |||
==Observations== | ==Observations== |
Revision as of 12:28, 5 February 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objective
Calculations
Procedure
Observations |