Talk:20.109(F13): Mod 1 Day 3 Agarose gel electrophoresis: Difference between revisions
No edit summary |
|||
| Line 30: | Line 30: | ||
===Pre-purification gels (for reference/posterity)=== | ===Pre-purification gels (for reference/posterity)=== | ||
Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page! | |||
[[Image:F13_M1D3_WF-Red.jpg|250px|thumb|right| Red Group]] | |||
[[Image:F13_M1D3_WF-Org.jpg|250px|thumb|left| Orange Group]] | |||
[[Image:F13_M1D3_WF-Ylw.jpg|250px|thumb|center| Yellow Group]] | |||
[[Image:F13_M1D3_WF-Grn.jpg|250px|thumb|right| Green Group]] | |||
[[Image:F13_M1D3_WF-Blu.jpg|250px|thumb|left| Blue Group]] | |||
[[Image:F13_M1D3_WF-Pnk.jpg|250px|thumb|center| Pink Group]] | |||
[[Image:F13_M1D3_WF-Prp.jpg|250px|thumb|left| Purple Group]] | |||
[[Image:F13_M1D3_WF-Whi.jpg|250px|thumb|center| White Group]] | |||
Revision as of 20:07, 18 September 2013
Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.
T/R lab
Here is your recovery gel: Error creating thumbnail: File missing
Top Gel (numbers refer to lanes):
- 1-2 Pink
- 3-4 Purple
- 5 MW
- 6-7 White
- 8-9 Blue
Bottom Gel (numbers refer to lanes):
- 1-2 Orange
- 3-4 Yellow
- 5 MW
- 6-7 Green
- 8-9 Platinum
- 10 Red
Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg).
W/F lab
Recovery gel
Pre-purification gels (for reference/posterity)
Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!
