Talk:20.109(F13): Mod 1 Day 3 Agarose gel electrophoresis: Difference between revisions
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==T/R lab== | ==T/R lab== | ||
===Recovery gel=== | |||
Here is your recovery gel: | Here is your recovery gel: | ||
[[Image:TR_F1320109_M1D3_recoverygel.BMP]] | [[Image:TR_F1320109_M1D3_recoverygel.BMP]] | ||
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Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg). | Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg). | ||
===Pre-purification gels (for reference/posterity)=== | |||
Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page! | |||
[[Image:F13_M1D3_TR-Red.jpg|250px|thumb|right| Red Group]] | |||
[[Image:F13_M1D3_TR-Org.jpg|250px|thumb|left| Orange Group]] | |||
[[Image:F13_M1D3_TR-Ylw.jpg|250px|thumb|center| Yellow Group]] | |||
[[Image:F13_M1D3_TR-Grn.jpg|250px|thumb|right| Green Group]] | |||
[[Image:F13_M1D3_TR-Blu.jpg|250px|thumb|left| Blue Group]] | |||
[[Image:F13_M1D3_TR-Pnk.jpg|250px|thumb|center| Pink Group]] | |||
[[Image:F13_M1D3_TR-Prp.jpg|250px|thumb|right| Purple Group]] | |||
[[Image:F13_M1D3_TR-Sil.jpg|250px|thumb|left| Silver Group]] | |||
[[Image:F13_M1D3_TR-Whi.jpg|250px|thumb|center| White Group]] | |||
==W/F lab== | ==W/F lab== | ||
Revision as of 20:09, 18 September 2013
Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.
T/R lab
Recovery gel
Here is your recovery gel: Error creating thumbnail: File missing
Top Gel (numbers refer to lanes):
- 1-2 Pink
- 3-4 Purple
- 5 MW
- 6-7 White
- 8-9 Blue
Bottom Gel (numbers refer to lanes):
- 1-2 Orange
- 3-4 Yellow
- 5 MW
- 6-7 Green
- 8-9 Platinum
- 10 Red
Note: Only a few groups recovered the digested plasmid. For your FNT assignment; Blue & Orange groups should do calculations based upon Pink backbone. Yellow & Green groups should do calculations based upon Purple backbone. Platinum & Red groups should do calculations based upon White backbone. Note: We loaded 20 μL of the solution that you left for us and 20 μL of MW marker was loaded (for a total 1.0 μg).
Pre-purification gels (for reference/posterity)
Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!
W/F lab
Recovery gel
Pre-purification gels (for reference/posterity)
Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!
