Biomod/2013/OSU/protocol/custom tem grids: Difference between revisions
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<div id="protocol-content" style="width:65%;margin-left:auto;margin-right:auto;"> | <div id="protocol-content" style="width:65%;margin-left:auto;margin-right:auto;"> | ||
<h1> | <h1>Operating TEM</h1> | ||
<h2> | <h2>Set-Up</h2> | ||
<ul> | <ul> | ||
<li> | <li>Make sure nitrogen tank is at least 2/3 full</li> | ||
<li> | <li>Make sure TEM is on - REd light and 2 white lights on</li> | ||
<li> | <li>Set high filament to 80 kV</li> | ||
<li>Make sure column valve is closed whenever putting sample in/out0</li> | |||
<li>vellum paper (Pergament-Papier)</li> | <li>vellum paper (Pergament-Papier)</li> | ||
<li> | <li>load grid sample side up, can tap to get in place</li> | ||
<li> | <li>then load with registry pin down. Turn about 5 degrees counter clock-wise. Vacuum should be automatic 50 seconds</li> | ||
<li> | <li>After around 50 seconds, red light goes off, turn the pin the rest of the 180 degrees and slowly guide into column</li> | ||
<li>To remove sample, pull out straight and then turn clockwise 180 degrees </li> | |||
<li>Once sample is in, open column valves and turn on filament </li> | |||
<li>Use intensity knob to spread beam </li> | |||
<li>Mouse ball to move stage </li> | |||
<li>Perform z axis wobbler to get image to stay in place </li> | |||
<li> Set locations on stage </li> | |||
<li> Set integrate time to about >4-5 seconds so you don't burn out the camera </li> | |||
<li> flip camera switch and cover viewing area. MAKE SURE THE CAMERA IS OUT AT ALL OTHER TIMES! </li> | |||
<li> Click for live image. use focus knob. set intensity to cover enter histogram to middle. FINAL IMAGE BABY </li> | |||
<li> Save it. </li> | |||
</ul> | </ul> | ||
</div> | </div> | ||
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{{OSUFooter}} | {{OSUFooter}} | ||
Latest revision as of 14:29, 26 October 2013
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<a href="http://openwetware.org/wiki/Biomod/2013"> << Back to Team Pages</a>
<img src="http://openwetware.org/images/1/15/Ohiomod2013.jpg" height="60%" width="60%" style="margin-left:-40px;">
</html>
<html>
Operating TEM
Set-Up
- Make sure nitrogen tank is at least 2/3 full
- Make sure TEM is on - REd light and 2 white lights on
- Set high filament to 80 kV
- Make sure column valve is closed whenever putting sample in/out0
- vellum paper (Pergament-Papier)
- load grid sample side up, can tap to get in place
- then load with registry pin down. Turn about 5 degrees counter clock-wise. Vacuum should be automatic 50 seconds
- After around 50 seconds, red light goes off, turn the pin the rest of the 180 degrees and slowly guide into column
- To remove sample, pull out straight and then turn clockwise 180 degrees
- Once sample is in, open column valves and turn on filament
- Use intensity knob to spread beam
- Mouse ball to move stage
- Perform z axis wobbler to get image to stay in place
- Set locations on stage
- Set integrate time to about >4-5 seconds so you don't burn out the camera
- flip camera switch and cover viewing area. MAKE SURE THE CAMERA IS OUT AT ALL OTHER TIMES!
- Click for live image. use focus knob. set intensity to cover enter histogram to middle. FINAL IMAGE BABY
- Save it.
</html>
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clear: both; /*may be omitted*/ position: absolute; bottom: 12px; background-color:#fffff; width: 100%; height: 40px; /* or anything you like */ }
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