IGEM:MIT/2006/Notebook/2007-1-27: Difference between revisions
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1. Dilute the osmY and control structures to 1:250 in 5 mL culture and grow up for 1 hr- DONE | 1. Dilute the osmY and control structures to 1:250 in 5 mL culture and grow up for 1 hr- DONE | ||
2. Run the osmY and control structures on the plate reader | 2. Run the osmY and control structures on the plate reader- DONE | ||
3. Make LCs of the three 170.320 colonies- DONE | 3. Make LCs of the three 170.320 colonies- DONE | ||
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4. Redigest 170- DONE | 4. Redigest 170- DONE | ||
5. Religate and transform 170.320 mix | 5. Religate and transform 170.320 mix- DONE | ||
6 | *Stephen - I put cells in warm room at 4:25...so plate after 5:25 I guess? I left plates out on the bench by the hot water bath. thanks! -Veena | ||
6. Run 170 cut on gel | |||
Latest revision as of 22:32, 27 January 2007
Plan
1. Dilute the osmY and control structures to 1:250 in 5 mL culture and grow up for 1 hr- DONE
2. Run the osmY and control structures on the plate reader- DONE
3. Make LCs of the three 170.320 colonies- DONE
4. Redigest 170- DONE
5. Religate and transform 170.320 mix- DONE
- Stephen - I put cells in warm room at 4:25...so plate after 5:25 I guess? I left plates out on the bench by the hot water bath. thanks! -Veena
6. Run 170 cut on gel
