User:Jhdavis: Difference between revisions
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#GFP Purification by organic extraction [[Jhdavis::GFPOrganic]] | #GFP Purification by organic extraction [[Jhdavis::GFPOrganic]] | ||
#Expression/Prep of ClpXdN-linked Trimers [[Jhdavis::ClpXPrep]] | #Expression/Prep of ClpXdN-linked Trimers [[Jhdavis::ClpXPrep]] | ||
#Peptide cleavage [[Jhdavis::PeptideCleavage]] | |||
#Flp recombinase [[Jhdavis::Flping]] | |||
#ClpP his prep from Kim 2000 [[Jhdavis::ClpPHisPrep]] | |||
#Sumo Protease expression/purification from Malakhov 2004 [[Jhdavis::SumoPrep]] | |||
#regenerating qiagen columns [[Jhdavis::regenQiagen]] | |||
#atpase notes [[Jhdavis::atpaseNotes]] | |||
#Immunoprecipitation from eyal (sherman et. al. 1992 EMBO J) [[Jhdavis::Immunoprecip]] | |||
==Buffers== | ==Buffers== | ||
#[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|PD Buffer]] | #[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|PD Buffer]] | ||
#[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|ATP Regeneration Mix]] | #[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|ATP Regeneration Mix]] | ||
#[[Sauer:Making_yeg_plates|YEG-Cl]] | |||
#[[Sauer:Davis Minimal Media]] | |||
#[[Jhdavis::4x reducing SDS-PAGE loading buffer]] | |||
==Info== | ==Info== | ||
*[[Jhdavis::GelFiltrationStandards]] | *[[Jhdavis::GelFiltrationStandards]] | ||
| Line 15: | Line 26: | ||
*[[Jhdavis::lacIoperators]] | *[[Jhdavis::lacIoperators]] | ||
*[[Jhdavis::DNA 1 letter codes]] | *[[Jhdavis::DNA 1 letter codes]] | ||
*[[Jhdavis::Useful pymol]] | |||
*[[Jhdavis::A260:A280 DNA:Protein ratios]] | |||
*[[Jhdavis::fplc singup]] | |||
==Links== | ==Links== | ||
Latest revision as of 18:26, 6 May 2009
I recently joined the Sauer Lab. clpX holla!!!!
Protocols
- Western Blots by Eyal Western::Eyal
- GFP Purification by organic extraction GFPOrganic
- Expression/Prep of ClpXdN-linked Trimers ClpXPrep
- Peptide cleavage PeptideCleavage
- Flp recombinase Flping
- ClpP his prep from Kim 2000 ClpPHisPrep
- Sumo Protease expression/purification from Malakhov 2004 SumoPrep
- regenerating qiagen columns regenQiagen
- atpase notes atpaseNotes
- Immunoprecipitation from eyal (sherman et. al. 1992 EMBO J) Immunoprecip
Buffers
Info
- GelFiltrationStandards
- MetCleavage
- lacIoperators
- DNA 1 letter codes
- Useful pymol
- A260:A280 DNA:Protein ratios
- fplc singup
