PrbbBB:Protocols: Difference between revisions
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* [[Prbbbb:fusion_biobrick_construction_v1|Ligation-free Biobrick Construction by PCR/recombination]] (RFC 25 [http://partsregistry.org/Help:Freiburg_standard Fusion format]) | * [[Prbbbb:fusion_biobrick_construction_v1|Ligation-free Biobrick Construction by PCR/recombination]] (RFC 25 [http://partsregistry.org/Help:Freiburg_standard Fusion format]) | ||
* [[Prbbbb:dna_measurement_on_plate_v1|DNA measurement on plates]] | * [[Prbbbb:dna_measurement_on_plate_v1|DNA measurement on plates]] | ||
* [[PrbbBB:Oligo_Annealing|Annealing of complementary long Oligos]] | |||
==Protein Expression== | ==Protein Expression== | ||
* [[Prbbbb:small_scale_expression_v1|Small-scale expression in BL21]] | * [[Prbbbb:small_scale_expression_v1|Small-scale expression in BL21]] | ||
* [[Prbbbb:large_scale_expression_v1|Large-scale expression in BL21]] | |||
* [[Prbbbb:inclusion_body_solubilization_v1|Solubilization of E. coli inclusion bodies]] | |||
* [[Prbbbb:Coomassi_Ethanol_stain|Coomassi protein stain (Ethanol-based)]] | |||
==Protein measurements== | |||
* [[Prbbbb:in_vitro_FRET_FRB_FKBP_v1|''in-vitro'' FRET measurements]] | |||
[[Category:Protocol]] | |||
Latest revision as of 13:11, 8 August 2010
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Biobrick assembly & related
We have streamlined the standard Biobrick assembly protocols. Our "Barcelona set" of protocols is optimized for performing many assemblies in parallel.
- prepare vector backbone for assembly (RFC 25 Fusion format)
- Assembly protocol for BioBricks on 96 well plate (RFC 25 Fusion format)
- Colony PCR screening of many assemblies in parallel (on 96 well plate)
- Ligation-free Biobrick Construction by PCR/recombination (RFC 25 Fusion format)
- DNA measurement on plates
- Annealing of complementary long Oligos
Protein Expression
- Small-scale expression in BL21
- Large-scale expression in BL21
- Solubilization of E. coli inclusion bodies
- Coomassi protein stain (Ethanol-based)
