User:Alexander Boehme/Notebook/BioE140L/Entry Base: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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PCA | |||
38 uL ddH2O | |||
5 ul 10x expand buffer | |||
5 ul 2mM dNTPs | |||
1 ul oligo mixture (100uM total, mixture of oligos after combination of 100uM stocks) | |||
0.75 ul Expand polymerase | |||
Program (can run JCA/PCA1) | |||
2 min initial denature at 94oC | |||
30 sec denature at 94oC | |||
30 sec anneal at 55oC [This should be the overlap temp of your oligos - vary as needed] | |||
30 sec extension at 72oC | |||
repeat 2-4 30 times total | |||
Latest revision as of 05:31, 27 September 2017
 Project name
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 Main project page |
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PCA 38 uL ddH2O 5 ul 10x expand buffer 5 ul 2mM dNTPs 1 ul oligo mixture (100uM total, mixture of oligos after combination of 100uM stocks) 0.75 ul Expand polymerase Program (can run JCA/PCA1) 2 min initial denature at 94oC 30 sec denature at 94oC 30 sec anneal at 55oC [This should be the overlap temp of your oligos - vary as needed] 30 sec extension at 72oC repeat 2-4 30 times total |
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