SDS: Difference between revisions
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'''SDS (sodium dodecyl sulfate/sulphate)''' is an anionic detergent effective in both acidic and alkaline solutions. SDS has a wide variety of applications, but is most often used in protein and lipid solubilisation. | '''SDS (sodium dodecyl sulfate/sulphate)''' is an anionic detergent effective in both acidic and alkaline solutions. SDS has a wide variety of applications, but is most often used in protein and lipid solubilisation. | ||
== SDS solubilisation == | |||
As a rule of thumb for the solubilisation/solubilization of proteins, SDS should be used at its critical micelle concentration (Womack'83 PMID 6882760). For the effects of SDS on protein conformation see Protein Structure by Creighton [http://books.google.de/books?id=KE-WlxzOxF0C&pg=PA349&lpg=PA349&dq=Protein+Structure:+A+Practical+Approach&source=bl&ots=G97vF5AaQ6&sig=m8YQBXGv8_wVKCs9_yr2wuJfqRc&hl=en&ei=VrTcSYLCM4SPsAan-LSmDA&sa=X&oi=book_result&ct=result]. For comparisons between SDS and other detergents for solubilisation of lipids, proteins, and its effect on | |||
enzymes activity see Womack '83 (PMID 6882760) and Creighton Protein Structure. | |||
Revision as of 14:34, 8 April 2009
SDS (sodium dodecyl sulfate/sulphate) is an anionic detergent effective in both acidic and alkaline solutions. SDS has a wide variety of applications, but is most often used in protein and lipid solubilisation.
SDS solubilisation
As a rule of thumb for the solubilisation/solubilization of proteins, SDS should be used at its critical micelle concentration (Womack'83 PMID 6882760). For the effects of SDS on protein conformation see Protein Structure by Creighton [1]. For comparisons between SDS and other detergents for solubilisation of lipids, proteins, and its effect on enzymes activity see Womack '83 (PMID 6882760) and Creighton Protein Structure.
